Autologous and not allogeneic adipose-derived stem cells improve acute burn wound healing.
Autologous and not allogeneic adipose-derived stem cells improve acute burn wound healing.
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DOI：
10.1371/journal.pone.0197744
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Lee SS
中科院分区：
文献类型：
--
作者：
Chang YW;Wu YC;Huang SH;Wang HD;Kuo YR;Lee SS
据报道，脂肪干细胞（ADSC）移植是治疗烧伤创面的潜在方法。然而，ADSCs的自体性和同种异体性对烧伤创面愈合的影响尚未被研究，ADSCs的使用方法仍需建立。本研究比较了自体和同种异体 ADSC 的愈合效果，并确定了使用 ADSC 治疗急性烧伤创面的最佳方法。实验在 20 只雄性 Wistar 大鼠（体重，176-250 克；年龄，6-7 周）中进行。每只大鼠都有两个相同的全层烧伤伤口（半径，4毫米）。将从腹股沟区域收获的具有高多能性的ADSCs注射到原始供体大鼠（自体ADSCs组）或其他大鼠（同种异体ADSCs组）的烧伤创面中。注射部位为伤口中心或距伤口边缘0.5cm的四个角。在受伤后15天内用Image J软件评估两个实验组和对照组烧伤表面积的减少，以确定伤口愈合率。与同种异体 ADSC 组 (p<0.05) 和对照组 (p<0.05) 相比，自体 ADSC 组的伤口愈合明显更快。同种异体 ADSC 组的伤口愈合与对照组没有显着差异。值得注意的是，与伤口中心注射 ADSC 相比，距离伤口边缘 0.5cm 处注射 ADSC 的愈合效果显着改善（p<0.05）。这项研究证明了 ADSC 对治疗大鼠急性烧伤创面的治疗效果。然而，只有自体 ADSC 才能改善急性烧伤创面的愈合；同种异体 ADSC 则没有。本研究进一步确定了使用ADSC注射治疗烧伤创面的优越部位，包括注射部位。未来的研究将在高等哺乳动物模型中在更大规模、更长期的烧伤创面中复制该实验，以促进 ADSC 在烧伤创面临床实践中的治疗。
Adipose-derived stem cells (ADSCs) transplant has been reported to be a potential treatment for burn wounds. However, the effects of autogenicity and allogenicity of ADSCs on burn wound healing have not been investigated and the method for using ADSCs still needs to be established. This study compared the healing effects of autologous and allogenic ADSCs and determined an optimal method of using ADSCs to treat acute burn wounds. Experiments were performed in 20 male Wistar rats (weight, 176–250 g; age, 6–7 weeks). Two identical full-thickness burn wounds (radius, 4 mm) were created in each rat. ADSCs harvested from inguinal area and characterized by their high multipotency were injected into burn wounds in the original donor rats (autologous ADSCs group) or in other rats (allogenic ADSCs group). The injection site was either the wound center or the four corners 0.5 cm from the wound edge. The reduction of burn surface areas in the two experimental groups and in control group were evaluated with Image J software for 15 days post-wounding to determine the wound healing rates. Wound healing was significantly faster in the autologous ADSCs group compared to both the allogenic ADSCs group (p<0.05) and control group (p<0.05). Wound healing in the allogenic ADSC group did not significantly differ from that in control group. Notably, ADSC injections 0.5cm from the wound edge showed significantly improved healing compared to ADSCs injections in the wound center (p<0.05). This study demonstrated the therapeutic efficacy of ADSCs in treating acute burn wounds in rats. However, only autologous ADSCs improved healing in acute burn wounds; allogenic ADSCs did not. This study further determined a superior location of using ADSCs injections to treat burn wounds including the injection site. Future studies will replicate the experiment in a larger and long-term scale burn wounds in higher mammalian models to facilitate ADSCs therapy in burn wound clinical practice.
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影响因子：
3.6
作者：
Lafosse, Aurore;Desmet, Celine;Dufrane, Denis
通讯作者：
Dufrane, Denis
影响因子：
0.7
作者：
Nikitina, V. A.;Osipova, E. Yu.;Bochkov, N. P.
通讯作者：
Bochkov, N. P.
影响因子：
5.6
作者：
Ojeh N;Pastar I;Tomic-Canic M;Stojadinovic O
通讯作者：
Stojadinovic O
影响因子：
2.7
作者：
Lee, SS;Lin, TM;Lai, CS
通讯作者：
Lai, CS
影响因子：
2.7
作者：
Lee, Su-Shin;Chen, Yi-Hui;Lai, Cheny-Sheng
通讯作者：
Lai, Cheny-Sheng