Analysis of angiotensin‐stimulated sodium transport in cultured smooth muscle cells from rat aorta
Analysis of angiotensin‐stimulated sodium transport in cultured smooth muscle cells from rat aorta
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大鼠主动脉培养平滑肌细胞中血管紧张素刺激的钠转运分析
DOI：
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发表时间：
1983
影响因子：
5.6
通讯作者：
T. Brock
中科院分区：
文献类型：
--
作者：
Jeffrey B. Smith;T. Brock
血管紧张素肽（AI， AII， AIII）增加了大鼠主动脉平滑肌细胞（SMC）的Na+积累速率。All对Na+摄取的刺激作用被观察到，通过Na+/K+泵的Na+流出被乌巴因或通过去除细胞外K+阻断。在刺激Na+摄取方面，所有这些都比AIII强至少10倍，比AI强约100倍。salalasin本身对Na+的摄取影响不大，但几乎完全阻断了All引起的Na+的增加。蛋白酶抑制剂可阻止AI而非AII刺激净Na+进入。阿米洛利几乎完全阻断了对Na+摄取的刺激。河豚毒素可以阻止缬草碱增加Na+的吸收，但对AII的反应没有影响。血管紧张素增加了瓦阿因敏感的86Rb+摄取率（Na+/K+泵活性），但对冷冻解冻SMC或培养SMC分离的微粒体膜中瓦阿因敏感的atp酶活性没有影响。salalasin阻断了All对瓦苦因敏感的86Rb+摄取的刺激。从外部介质中省略Na+阻止All增加86Rb+的摄取。所有这些都对培养SMC的细胞体积或循环AMP水平没有影响。这些结果表明，血管紧张素肽激活了一个对氨酰敏感的Na+转运体，该转运体为Na+ /K+泵提供更多的Na+，这是其限速底物。
Angiotensin peptides (AI, AII, AIII) increased the rate of Na+ accumulation by smooth muscle cells (SMC) cultured from rat aorta. The stimulatory effect of All on Na+ uptake was observed which Na+ exodus via the Na+/K+ pump was blocked either by ouabain or by the removal of extracellular K+. All was at least ten times more potent than AIII and about 100 times more potent than AI in stimulating Na+ uptake. Saralasin had little effect on Na+ uptake by itself but almost completely blocked the increase caused by All. The stimulation of net Na+ entry by AI, but not AII, was prevented by protease inhibitors. The stimulation of Na+ uptake was almost completely blocked by amiloride. Tetrodotoxin, which prevented veratridine from increasing Na+ uptake, had no effect on the response to AII. Angiotensin increased the rate of ouabain‐sensitive 86Rb+ uptake (Na+/K+ pump activity) but had no effect on ouabain‐sensitive ATPase activity in frozen‐thawed SMC or in microsomal membranes isolated from cultured SMC. The stimulation of ouabain‐sensitive 86Rb+ uptake by All was blocked by saralasin. Omitting Na+ from the external medium prevented All from increasing 86Rb+ uptake. All had no effect on cell volume or cyclic AMP levels in the cultured SMC. These results suggest that angiotensin peptides activate an amiloride‐sensitive Na+ transporter which supplies the Na+ /K+ pump with more Na+, its rate‐limiting substrate.
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血管平滑肌培养物中莫能菌素对 Na /K 泵的可逆刺激。
影响因子：
6.1
作者：
Brock,TA;Smith,JB
通讯作者：
Smith,JB
异丙肾上腺素、二丁酰环 AMP 和茶碱可松弛血管平滑肌。
DOI：
--
发表时间：
1981
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Webb,RC;Bohr,DF
通讯作者：
Bohr,DF
血管紧张素 II-铁蛋白缀合物对兔主动脉平滑肌细胞的生物活性。
影响因子：
2.9
作者：
Schultz,GS;Galardy,RE;Jamieson,JD
通讯作者：
Jamieson,JD
血管紧张素诱导培养的大鼠主动脉平滑肌细胞重新聚集体的主动反应。
DOI：
10.1159/000158360
发表时间：
1981
期刊：
Blood vessels
影响因子：
--
作者：
Zelcer,E;Sperelakis,N
通讯作者：
Sperelakis,N