The rat alpha-tropomyosin gene generates a minimum of six different mRNAs coding for striated, smooth, and nonmuscle isoforms by alternative splicing
The rat alpha-tropomyosin gene generates a minimum of six different mRNAs coding for striated, smooth, and nonmuscle isoforms by alternative splicing
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大鼠 α-原肌球蛋白基因通过选择性剪接产生至少六种不同的 mRNA，编码横纹肌、平滑肌和非肌肉亚型
DOI：
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发表时间：
1988
影响因子：
5.3
通讯作者：
B. N. S. Jzumo
中科院分区：
文献类型：
--
作者：
David F. Wieczorek;Christopher W. J. Smith;B. N. Ginard;B. N. S. Jzumo
原肌球蛋白（TM）是一种普遍存在的蛋白质，是所有细胞收缩装置的组成部分。在非肌肉细胞中，它存在于应力纤维中，而在肌节和非肌节肌肉中，它是细丝的组成部分。已经描述了对非肌细胞和不同类型的肌细胞具有特异性的几种不同的TM同种型。至于其他收缩蛋白质，人们认为平滑肌、横纹肌和非肌肉的同种型都是由不同的基因编码的。通过使用S1核酸酶作图，RNA印迹和5'延伸分析，我们表明，大鼠alpha-TM基因，其表达，直到现在被认为是限制在肌肉细胞，产生许多不同的组织特异性亚型。该基因的启动子在其利用模式（在大多数细胞类型中具有活性）和其缺乏任何典型序列元件方面似乎与其他管家启动子非常相似。大鼠α-TM基因被分成至少13个外显子，其中7个外显子以组织特异性方式选择性剪接。这种基因排列也包括两个不同的3'末端，产生至少六种不同的mRNA，每种mRNA都具有编码不同蛋白质的能力。这些不同的TM亚型在非肌肉和平滑肌和横纹肌（心肌和骨骼肌）细胞中特异性表达。因此，这些异构体的组织特异性表达和发育调节是由替代mRNA加工产生的。此外，来自不同门的TM基因之间的结构和序列比较表明，选择性剪接是进化上一个非常古老的事件，在基因进化中发挥了重要作用，并可能出现伴随甚至之前组成性剪接。
Tropomyosin (TM), a ubiquitous protein, is a component of the contractile apparatus of all cells. In nonmuscle cells, it is found in stress fibers, while in sarcomeric and nonsarcomeric muscle, it is a component of the thin filament. Several different TM isoforms specific for nonmuscle cells and different types of muscle cell have been described. As for other contractile proteins, it was assumed that smooth, striated, and nonmuscle isoforms were each encoded by different sets of genes. Through the use of S1 nuclease mapping, RNA blots, and 5' extension analyses, we showed that the rat alpha-TM gene, whose expression was until now considered to be restricted to muscle cells, generates many different tissue-specific isoforms. The promoter of the gene appears to be very similar to other housekeeping promoters in both its pattern of utilization, being active in most cell types, and its lack of any canonical sequence elements. The rat alpha-TM gene is split into at least 13 exons, 7 of which are alternatively spliced in a tissue-specific manner. This gene arrangement, which also includes two different 3' ends, generates a minimum of six different mRNAs each with the capacity to code for a different protein. These distinct TM isoforms are expressed specifically in nonmuscle and smooth and striated (cardiac and skeletal) muscle cells. The tissue-specific expression and developmental regulation of these isoforms is, therefore, produced by alternative mRNA processing. Moreover, structural and sequence comparisons among TM genes from different phyla suggest that alternative splicing is evolutionarily a very old event that played an important role in gene evolution and might have appeared concomitantly with or even before constitutive splicing.
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致瘤性人成纤维细胞中原肌球蛋白异构体的转换。
影响因子：
5.3
作者：
Leavitt,J;Latter,G;Lutomski,L;Goldstein,D;Burbeck,S
通讯作者：
Burbeck,S
从培养细胞中分离和表征含有原肌球蛋白的微丝。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Matsumura,F;Yamashiro-Matsumura,S;Lin,JJ
通讯作者：
Lin,JJ
原肌球蛋白原子模型的构建及其与肌动蛋白相互作用的影响。
影响因子：
5.6
作者：
PhillipsJr,GN
通讯作者：
PhillipsJr,GN
来自鸡脑和肠上皮的低 Mr 原肌球蛋白亚型具有独特的肌动蛋白结合特性。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Broschat,KO;Burgess,DR
通讯作者：
Burgess,DR