Prospective identification and skeletal localization of cells capable of multilineage differentiation in vivo.
Prospective identification and skeletal localization of cells capable of multilineage differentiation in vivo.
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DOI：
10.1089/scd.2009.0445
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发表时间：
2010-10
影响因子：
4
通讯作者：
Krebsbach PH
中科院分区：
文献类型：
--
作者：
Taichman RS;Wang Z;Shiozawa Y;Jung Y;Song J;Balduino A;Wang J;Patel LR;Havens AM;Kucia M;Ratajczak MZ;Krebsbach PH
一个前瞻性的体内试验被用来鉴定具有多向分化潜能的细胞。本实验首次确定了体内能形成骨组织的5-FU耐药细胞在体外也向SDF-1迁移。同时，采用一种基于荧光激活细胞分选的分离方法对−Sca-1+CD45−细胞中的极小细胞胚胎样细胞进行了鉴定，该细胞在体内能够形成骨样结构。不同的骨髓分离研究表明，大多数LIN−SCA-1+CD45CD45−细胞位于骨髓的骨膜下区域。为了确定这些细胞是否能够分化为多个谱系，从Col2.3ΔTK小鼠中获取的基质细胞被植入到SCID小鼠体内，以产生胸苷激酶敏感的听骨。1.5个月后，将2000GFP+LIN−Sca-1+CD45CD45−细胞注入听小骨。收获时，观察绿色荧光蛋白表达细胞与成骨细胞特异性标志物RUNX-2和脂肪细胞标志物PPARγ抗体的共定位。根据未培养的细胞在体内分化为多个间充质系的能力以及在低密度下生成骨组织的能力，我们认为这种细胞群符合间充质干细胞的许多特征。
A prospective in vivo assay was used to identify cells with potential for multiple lineage differentiation. With this assay it was first determined that the 5-FU resistant cells capable of osseous tissue formation in vivo also migrated towards SDF-1 in vitro. In parallel, an isolation method based upon fluorescence activated cell sorting (FACS) was employed to identify a very small cell embryonic-like (VSEL) Lin−Sca-1+ CD45− cell that with as few as 500 cells were capable of forming bone-like structures in vivo. Differential marrow fractionation studies determined that the majority of the Lin−Sca-1+CD45− cells reside in the subendosteal regions of marrow. To determine if these cells were capable of differentiating into multiple lineages, stromal cells harvested from Col2.3ΔTK mice were implanted with a gelatin sponge into SCID mice to generate thymidine kinase sensitive ossicles. At 1.5 months, 2000 GFP+ Lin−Sca-1+ CD45− cells were injected into the ossicles. At harvest, co-localization of GFP expressing cells with antibodies to the osteoblast specific marker Runx-2 and the adipocyte marker PPARγ were observed. Based upon the ability of the non-cultured cells to differentiate into multiple mesenchymal lineages in vivo and the ability to generate osseous tissues at low density, we propose that this population fulfills many of the characteristics of mesenchymal stem cells.
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影响因子：
2.6
作者：
Mankani, Mahesh H.;Kuznetsov, Sergei A.;Robey, Pamela Gehron
通讯作者：
Robey, Pamela Gehron
影响因子：
2.8
作者：
Schneider, A;Taboas, JM;Krebsbach, PH
通讯作者：
Krebsbach, PH
影响因子：
1.2
作者：
Kuznetsov, Sergei A.;Mankani, Mahesh H.;Bianco, Paolo;Robey, Pamela G.
通讯作者：
Robey, Pamela G.
影响因子：
19.7
作者：
Mankani, MH;Kuznetsov, SA;Robey, PG
通讯作者：
Robey, PG
影响因子：
20.3
作者：
Aggarwal, S;Pittenger, MF
通讯作者：
Pittenger, MF