Recombinant Bacillus anthracis spore proteins enhance protection of mice primed with suboptimal amounts of protective antigen.
Recombinant Bacillus anthracis spore proteins enhance protection of mice primed with suboptimal amounts of protective antigen.
复制标题
重组炭疽芽孢杆菌孢子蛋白增强了对次优量保护性抗原引发的小鼠的保护。
DOI：
10.1016/j.vaccine.2008.07.015
复制
发表时间：
2008-09-08
期刊：
影响因子：
5.5
通讯作者：
O'Brien, Alison D.
中科院分区：
文献类型：
--
作者：
Cybulsk, Robert J., Jr.;Sanza, Patrick;McDaniel, Dennis;Darnell, Steve;Bull, Robert L.;O'Brien, Alison D.
灭活的炭疽芽孢杆菌孢子与保护性抗原（PA）一起给予有助于在几种动物模型中对炭疽的免疫。针对整个辐照B产生的抗血清。炭疽孢子已经显示出在体外具有抗萌发和调理活性。基于这些观察，我们假设表面暴露的孢子蛋白可能作为PA为基础的炭疽疫苗的补充成分。测试保护性抗孢子血清与已知或认为存在于B内的30种蛋白质的重组形式的反应性。炭疽病孢子外壁。这些蛋白质中的11种与该抗血清反应，并且随后使用该组的子集来产生兔多克隆抗体。评价这些血清对Sterne菌株产生的完整孢子以及缺乏孢子表面蛋白芽孢杆菌胶原样抗原（BclA）的同基因突变体产生的完整孢子上的免疫原的识别。这些数据与所讨论的抗原位于外孢壁基底表面上的BclA下方的概念一致。A/J小鼠免疫的假设蛋白p5303或结构蛋白BxpB，每一个与PA的亚保护水平的组合，表现出增强的保护对皮下孢子的挑战。虽然抗BxpB或抗p5303抗体都没有降低体外孢子萌发率，但都引起摄取增加并导致吞噬细胞的破坏率更高。我们的结论是，通过促进更有效的吞噬清除孢子，抗体对个别孢子外壁组件可以有助于保护对B。炭疽感染。
Inactivated Bacillus anthracis spores given with protective antigen (PA) contribute to immunity against anthrax in several animal models. Antiserum raised against whole irradiated B. anthracis spores has been shown to have anti-germination and opsonic activities in vitro. Based on these observations, we hypothesized that surface-exposed spore proteins might serve as supplemental components of a PA-based anthrax vaccine. The protective anti-spore serum was tested for reactivity with recombinant forms of 30 proteins known, or believed to be, present within the B. anthracis exosporium. Eleven of those proteins were reactive with this antiserum, and, subsequently a subset of this group was used to generate rabbit polyclonal antibodies. These sera were evaluated for recognition of the immunogens on intact spores generated from Sterne strain, as well as from an isogenic mutant lacking the spore surface protein Bacillus collagen-like antigen (BclA). The data were consistent with the notion that the antigens in question were located beneath BclA on the basal surface of the exosporium. A/J mice immunized with either the here-to-for hypothetical protein p5303 or the structural protein BxpB, each in combination with subprotective levels of PA, showed enhanced protection against subcutaneous spore challenge. While neither anti-BxpB or anti-p5303 antibodies reduced the rate of spore germination in vitro, both caused increased uptake and lead to a higher rate of destruction by phagocytic cells. We conclude that by facilitating more efficient phagocytic clearance of spores, antibodies against individual exosporium components can contribute to protection against B. anthracis infection.
登录
查看更多内容
影响因子：
2.8
作者：
Enkhtuya, Jargalsaikhan;Kawamoto, Keiko;Makino, Sou-ichi
通讯作者：
Makino, Sou-ichi
影响因子：
5.5
作者：
Hahn, UK;Boehm, R;Beyer, W
通讯作者：
Beyer, W
影响因子：
3.1
作者：
Brahmbhatt, Trupti N.;Janes, Brian K.;O'Brien, Alison D.
通讯作者：
O'Brien, Alison D.
影响因子：
3.1
作者：
Cohen, S;Mendelson, I;Shafferman, A
通讯作者：
Shafferman, A
影响因子：
4
作者：
Charlton, S;Moir, AJG;Moir, A
通讯作者：
Moir, A