Isolation and characterization of new Botryococcus braunii (Trebouxiophyceae) isolates
Isolation and characterization of new Botryococcus braunii (Trebouxiophyceae) isolates
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新布氏葡萄球菌(Trebouxiophyceae)分离株的分离和表征
DOI:
10.1016/j.renene.2019.04.048
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发表时间:
2019
期刊:
影响因子:
8.7
通讯作者:
Abdel-Hamid M
中科院分区:
文献类型:
--
作者:
Abdel-Hamid M
Four isolates ofBotryococcus brauniiKützing were isolated from the Nile River in Egypt. The morphological examinations and results of 5.8S rRNA gene sequences confirmed that EG-Bb01 (JN580448.1), EG-Bb02 (JN580449.1), EG-Bb03 (JN580450.1) and EG-Bb04 (JN580451.1) areB. braunii. The isolates were cultivated in modified Chu 13 medium and incubated for 8 weeks at 25±1 °C under light intensity of 16 ± 3 μmol m−2s−1with 16:8 h light: dark cycle. The dry weight biomass ranged between 0.382 gl−1and 0.628 gl−1for the isolates EG-Bb03 and EG-Bb01. Also, the hydrocarbon production (% w/w) varied significantly (P≤ 0.05) between 33% (EG-Bb03) and 53% (EG-Bb01). GC/MS analysis revealed marked qualitative and quantitative variations in hydrocarbon profile ofB. brauniiisolates. A total of 24 C16single bondC31different compounds were identified. The hydrocarbon of the four isolates was mainly composed of aliphatic hydrocarbons with the odd carbon numbered C25single bondC31, indicating that these isolates are a typical race A ofB. braunii. Isolate EG-Bb01 maintained the highest content of C25single bondC31hydrocarbons of (36.44%). The hexahydrofarnesylacetone (C18H38O) ketone was detected in the four isolates with different proportions ranged between 0.216% (EG-Bb01) and 13.86% (EG-Bb04). Meanwhile, the alcohol hexacosanol C26H54O (31.52%) was only detected in the isolate EG-Bb03.
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DOI:
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发表时间:
1988
期刊:
影响因子:
--
作者:
M. David;P. Metzger;E. Casadevall
通讯作者:
E. Casadevall
DOI:
--
发表时间:
1988
期刊:
影响因子:
--
作者:
Zhengui Huang;C. Poulter;F. Wolf;T. Somers;James D White
通讯作者:
James D White
影响因子:
1.4
作者:
T. Volova;G. Kalacheva;N. Zhila
通讯作者:
N. Zhila
DOI:
--
发表时间:
1968
期刊:
Archiv für Mikrobiologie
影响因子:
--
作者:
J. Belcher
通讯作者:
J. Belcher
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
S. Rayne
通讯作者:
S. Rayne