Isolation and characterization of new Botryococcus braunii (Trebouxiophyceae) isolates

Isolation and characterization of new Botryococcus braunii (Trebouxiophyceae) isolates
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新布氏葡萄球菌(Trebouxiophyceae)分离株的分离和表征

DOI:
10.1016/j.renene.2019.04.048
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发表时间:
2019
期刊:
影响因子:
8.7
通讯作者:
Abdel-Hamid M
Abdel-Hamid M
中科院分区:
工程技术1区
文献类型:
--
作者:
Abdel-Hamid M

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从埃及尼罗河中分离到 4 株布氏葡萄球菌 Kützing。形态学检查和5.8S rRNA基因序列结果证实EG-Bb01(JN580448.1)、EG-Bb02(JN580449.1)、EG-Bb03(JN580450.1)和EG-Bb04(JN580451.1)为B。 布劳尼。将分离株培养在改良的 Chu 13 培养基中,并在 25±1°C、光强度 16±3μmolm−2s−1、16:8h 光:暗循环下孵育 8 周。分离株 EG-Bb03 和 EG-Bb01 的干重生物量范围在 0.382gl−1 和 0.628gl−1 之间。此外,烃产量(%w/w)在33%(EG-Bb03)和53%(EG-Bb01)之间显着变化(P≤0.05)。 GC/MS 分析揭示了 B 烃谱的显着定性和定量变化。 布劳尼隔离。共鉴定出24个C16单键C31不同的化合物。 4个分离物的烃主要由奇数碳原子数为C25单键C31的脂肪族烃组成,表明这些分离物是典型的A of B小种。 布劳尼。分离株EG-Bb01保持了最高的C25单键C31烃含量(36.44%)。在四个分离株中检测到六氢法呢基丙酮(C18H38O)酮,其比例范围在0.216%(EG-Bb01)和13.86%(EG-Bb04)之间。同时,仅在分离株EG-Bb03中检测到醇二十六烷醇C26H54O(31.52%)。
Four isolates ofBotryococcus brauniiKützing were isolated from the Nile River in Egypt. The morphological examinations and results of 5.8S rRNA gene sequences confirmed that EG-Bb01 (JN580448.1), EG-Bb02 (JN580449.1), EG-Bb03 (JN580450.1) and EG-Bb04 (JN580451.1) areB. braunii. The isolates were cultivated in modified Chu 13 medium and incubated for 8 weeks at 25±1 °C under light intensity of 16 ± 3 μmol m−2s−1with 16:8 h light: dark cycle. The dry weight biomass ranged between 0.382 gl−1and 0.628 gl−1for the isolates EG-Bb03 and EG-Bb01. Also, the hydrocarbon production (% w/w) varied significantly (P≤ 0.05) between 33% (EG-Bb03) and 53% (EG-Bb01). GC/MS analysis revealed marked qualitative and quantitative variations in hydrocarbon profile ofB. brauniiisolates. A total of 24 C16single bondC31different compounds were identified. The hydrocarbon of the four isolates was mainly composed of aliphatic hydrocarbons with the odd carbon numbered C25single bondC31, indicating that these isolates are a typical race A ofB. braunii. Isolate EG-Bb01 maintained the highest content of C25single bondC31hydrocarbons of (36.44%). The hexahydrofarnesylacetone (C18H38O) ketone was detected in the four isolates with different proportions ranged between 0.216% (EG-Bb01) and 13.86% (EG-Bb04). Meanwhile, the alcohol hexacosanol C26H54O (31.52%) was only detected in the isolate EG-Bb03.
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