Integration of semiconductor quantum dots into nano-bio-chip systems for enumeration of CD4+ T cell counts at the point-of-need.
Integration of semiconductor quantum dots into nano-bio-chip systems for enumeration of CD4+ T cell counts at the point-of-need.
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DOI：
10.1039/b817116e
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发表时间：
2008-12
期刊：
影响因子：
6.1
通讯作者：
McDevitt JT
中科院分区：
文献类型：
--
作者：
Jokerst JV;Floriano PN;Christodoulides N;Simmons GW;McDevitt JT
最近的人道主义努力导致广泛发放抗逆转录病毒药物，用于治疗生活在资源匮乏环境中的3300多万受艾滋病毒困扰的人。在这里，需要对CD4+T淋巴细胞进行计数，以确定免疫系统受到损害的程度。发达国家使用的基于流式细胞术的金标准方法虽然被广泛接受和准确，但由于费用高、技术要求高、操作维护困难以及这些复杂的实验室限制系统缺乏可移植性，因此无法在资源稀缺的环境中广泛使用。作为开发实用诊断仪器的持续努力的一部分，已完成将半导体纳米晶体(量子点、量子点)集成到便携式微流控淋巴细胞捕获和检测设备中。该集成系统能够从全血样本中分离和计数选定的淋巴细胞亚群(CD3+CD4+)。通过将量子点的独特光学性质与新型微流控系统的样品处理能力和成本效益相结合，已经开发出一种实用的便携式淋巴细胞测量方法，该方法与流式细胞术(R2=0.97)具有很好的相关性。这种基于QD的系统大大降低了相对于分子荧光团的光学要求，并且迷你CD4计数设备预计适用于需求点和资源稀缺的环境。
Recent humanitarian efforts have led to the widespread release of antiretroviral drugs for the treatment of the more than 33 million HIV afflicted people living in resource-scarce settings. Here, the enumeration of CD4+ T lymphocytes is required to establish the level at which the immune system has been compromised. The gold standard method used in developed countries, based on flow cytometry, though widely accepted and accurate, is precluded from widespread use in resource-scarce settings due to its high expense, high technical requirements, difficulty in operation-maintenance and the lack of portability for these sophisticated laboratory-confined systems. As part of continuing efforts to develop practical diagnostic instrumentation, the integration of semiconductor nanocrystals (quantum dots, QDs) into a portable microfluidic-based lymphocyte capture and detection device is completed. This integrated system is capable of isolating and counting selected lymphocyte sub-populations (CD3+CD4+) from whole blood samples. By combining the unique optical properties of the QDs with the sample handling capabilities and cost effectiveness of novel microfluidic systems, a practical, portable lymphocyte measurement modality that correlates nicely with flow cytometry (R2 = 0.97) has been developed. This QD-based system reduces the optical requirements significantly relative to molecular fluorophores and the mini-CD4 counting device is projected to be suitable for use in both point-of-need and resource-scarce settings.
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DOI：
10.1007/10_2007_069
发表时间：
2007-01-01
期刊：
CELL SEPARATION: FUNDAMENTALS, ANALYTICAL AND PREPARATIVE METHODS
影响因子：
--
作者：
Dainiak, Maria B.;Kumar, Ashok;Mattiasson, Bo
通讯作者：
Mattiasson, Bo
影响因子：
15
作者：
Liu, Wenhao;Howarth, Mark;Bawendi, Moungi G.
通讯作者：
Bawendi, Moungi G.
影响因子：
9.3
作者：
Christodoulides, N;Floriano, PN;McDevitt, JT
通讯作者：
McDevitt, JT
影响因子：
15
作者：
Lavigne, JJ;Savoy, S;Neikirk, D
通讯作者：
Neikirk, D
影响因子：
15
作者：
Liu, Wenhao;Choi, Hak Soo;Bawendi, Moungi
通讯作者：
Bawendi, Moungi