Evolution and expression analyses of the MADS-box gene family in Brassica napus.
Evolution and expression analyses of the MADS-box gene family in Brassica napus.
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甘蓝型油菜MADS-box基因家族的进化和表达分析
DOI：
10.1371/journal.pone.0200762
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Du H
中科院分区：
文献类型：
--
作者：
Wu Y;Ke Y;Wen J;Guo P;Ran F;Wang M;Liu M;Li P;Li J;Du H
MADS-box转录因子对植物生长发育具有重要意义，目前已有数百个MADS-box基因在植物中得到功能鉴定。然而，对这些基因在经济上重要的异源多倍体油料作物甘蓝型油菜中的功能知之甚少。我们从油菜基因组中鉴定出307个潜在的MADS-box基因（bnmads），并根据系统发育、蛋白基序结构和外显子-内含子组织将它们分为I型（Mα、Mβ和Mγ）和II型（MADS dna结合域、干预域、角蛋白样域和c -末端域[MIKC]c和MIKC*）。我们在MIKCc基因的MADS-box结构域中发现了一个保守的内含子模式，在K-box结构域中发现了七个保守的内含子模式，这些模式以前被忽视，可能与功能有关。染色体分布和同源性分析表明，油菜和甘蓝的杂交、片段重复和同源交换（HE）是油菜bnmads扩增的主要机制。启动子顺式元件分析表明，bnmads可能对各种胁迫（干旱、热、激素）和光产生响应。表达分析表明，在给定的亚家族或姐妹对中同源基因高度保守，表明广泛的功能保守和冗余。分析bnmads为了解其在植物发育中的功能作用提供了基础。
MADS-box transcription factors are important for plant growth and development, and hundreds of MADS-box genes have been functionally characterized in plants. However, less is known about the functions of these genes in the economically important allopolyploid oil crop, Brassica napus. We identified 307 potential MADS-box genes (BnMADSs) in the B. napus genome and categorized them into type I (Mα, Mβ, and Mγ) and type II (MADS DNA-binding domain, intervening domain, keratin-like domain, and C-terminal domain [MIKC]c and MIKC*) based on phylogeny, protein motif structure, and exon-intron organization. We identified one conserved intron pattern in the MADS-box domain and seven conserved intron patterns in the K-box domain of the MIKCc genes that were previously ignored and may be associated with function. Chromosome distribution and synteny analysis revealed that hybridization between Brassica rapa and Brassica oleracea, segmental duplication, and homologous exchange (HE) in B. napus were the main BnMADSs expansion mechanisms. Promoter cis-element analyses indicated that BnMADSs may respond to various stressors (drought, heat, hormones) and light. Expression analyses showed that homologous genes in a given subfamily or sister pair are highly conserved, indicating widespread functional conservation and redundancy. Analyses of BnMADSs provide a basis for understanding their functional roles in plant development.
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大米中的MADS-box基因家族：在生殖发育和压力期间，全基因组识别，组织和表达分析。
影响因子：
4.4
作者：
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通讯作者：
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影响因子：
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通讯作者：
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影响因子：
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