The smooth muscle cell. I. In vivo synthesis of connective tissue proteins.
The smooth muscle cell. I. In vivo synthesis of connective tissue proteins.
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DOI：
10.1083/jcb.50.1.159
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发表时间：
1971-07
期刊：
影响因子：
--
通讯作者：
Klebanoff SJ
中科院分区：
文献类型：
--
作者：
Ross R;Klebanoff SJ
这些研究检测了青春期前大鼠主动脉发育中的平滑肌细胞利用氨基酸合成和分泌结缔组织蛋白的能力。前青春期大鼠，先前给予酒精载体或雌二醇-17- β，每只给予静脉注射脯氨酸- 3h。15 min、30 min和4 h后处死。主动脉平滑肌和子宫内膜细胞的光镜和电子显微镜放射照片显示，两组动物的主动脉细胞和雌激素刺激动物的子宫内膜细胞都吸收脯氨酸，并在4小时内迅速将脯氨酸分泌到胶原和弹性纤维中。相比之下，未受刺激动物的子宫肌瘤细胞吸收了相对少量的脯氨酸，并保留了细胞内的大部分氨基酸。电镜放射照片显示，参与这种活动的细胞器是粗内质网和高尔基复合体以及周围元素，推测为小泡。这些研究表明，发育中的主动脉和雌激素刺激的肌层的平滑肌细胞具有合成和分泌与细胞外结缔组织基质相关的蛋白质的能力。
These studies have examined the ability of smooth muscle cells from developing aorta of the prepubertal rat to utilize amino acids in the synthesis and secretion of connective tissue proteins. Prepubertal rats, previously given either an alcohol carrier or estradiol-17-beta, were each given an intravenous injection of proline-3H. The animals were sacrificed after 15 and 30 min, and 4 hr. Light and electron microscope radioautographs of the aortic smooth muscle and of the myometrial cells demonstrated that the aortic cells, in both groups of animals, and the myometrial cells, in the estrogen-stimulated animals, took up the proline and rapidly secreted it in both collagen and elastic fibers within 4 hr. In contrast, the myometrial cells of the nonstimulated animal took up relatively small amounts of proline and retained most of the amino acid within the cells. Electron microscope radioautographs demonstrated that the organelles involved in this activity were the rough endoplasmic reticulum and Golgi complex together with peripheral elements, presumed to be small vesicles. These studies have demonstrated that the smooth muscle cells of the developing aorta and of the estrogen-stimulated myometrium have a capacity to synthesize and secrete proteins associated with the extracellular connective tissue matrix.
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DOI：
10.1083/jcb.36.3.639
发表时间：
1968-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bergman RA
通讯作者：
Bergman RA
影响因子：
--
作者：
BO, WJ;ODOR, DL;ROTHROCK, M
通讯作者：
ROTHROCK, M
影响因子：
3.2
作者：
KOPRIWA, BM
通讯作者：
KOPRIWA, BM
影响因子：
3.6
作者：
IMAI, H;LEE, KT;THOMAS, WA
通讯作者：
THOMAS, WA
DOI：
10.1016/s0022-5320(61)80002-6
发表时间：
1961-01-01
期刊：
JOURNAL OF ULTRASTRUCTURE RESEARCH
影响因子：
--
作者：
KARRER, HE
通讯作者：
KARRER, HE