Sperm protein 17 is expressed in human nervous system tumours.
Sperm protein 17 is expressed in human nervous system tumours.
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精子蛋白17在人类神经系统肿瘤中表达。
DOI：
10.1186/1471-2407-6-23
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发表时间：
2006-01-26
期刊：
影响因子：
3.8
通讯作者：
Chiriva-Internati, M
中科院分区：
文献类型：
--
作者：
Grizzi, F;Gaetani, P;Franceschini, B;Di Ieva, A;Colombo, P;Ceva-Grimaldi, G;Bollati, A;Frezza, EE;Cobos, E;Baena, RRY;Dioguardi, N;Chiriva-Internati, M
人精子蛋白17是一种高度保守的蛋白质，最初从兔附睾精子膜和睾丸膜颗粒中分离出来。它最近被列入癌症/睾丸(CT)抗原家族，并被证明在多发性骨髓瘤和卵巢癌中表达。我们研究了它在神经系统(NS)恶性肿瘤标本中的免疫定位，以确定它作为肿瘤疫苗策略的靶点的有效性。采用标准免疫组织化学方法[(mAb/抗原)MF1/Sp17]，对28例神经外胚层原发肿瘤(星形细胞瘤6例，多形性胶质母细胞瘤16例，少突胶质细胞瘤5例，室管膜瘤1例)、25例脑膜肿瘤和5例周围神经鞘肿瘤(4例神经鞘瘤和1例神经纤维瘤)进行了检测。许多神经外胚层(21%)和脑膜肿瘤(4%)被发现异质性免疫阳性。周围神经鞘瘤中无一例SP17免疫阳性。所有阳性标本的表达模式都是异质性的，且与恶性程度无关。Sp17的表达频率和细胞分布的不均一性表明，它不能作为NS癌唯一的免疫治疗靶点。然而，我们的结果确实显示了Sp17在一定比例的NS肿瘤细胞中的免疫定位，但在它们的非病理对应细胞中没有。新出现的Sp17的复杂功能使得进一步研究它与免疫阳性细胞之间的联系是必要的。
Human sperm protein 17 (Sp17) is a highly conserved protein that was originally isolated from a rabbit epididymal sperm membrane and testis membrane pellet. It has recently been included in the cancer/testis (CT) antigen family, and shown to be expressed in multiple myeloma and ovarian cancer. We investigated its immunolocalisation in specimens of nervous system (NS) malignancies, in order to establish its usefulness as a target for tumour-vaccine strategies. The expression of Sp17 was assessed by means of a standardised immunohistochemical procedure [(mAb/antigen) MF1/Sp17] in formalin-fixed and paraffin embedded surgical specimens of NS malignancies, including 28 neuroectodermal primary tumours (6 astrocytomas, 16 glioblastoma multiforme, 5 oligodendrogliomas, and 1 ependymoma), 25 meningeal tumours, and five peripheral nerve sheath tumours (4 schwannomas, and 1 neurofibroma),. A number of neuroectodermal (21%) and meningeal tumours (4%) were found heterogeneously immunopositive for Sp17. None of the peripheral nerve sheath tumours was immunopositive for Sp17. The expression pattern was heterogeneous in all of the positive samples, and did not correlate with the degree of malignancy. The frequency of expression and non-uniform cell distribution of Sp17 suggest that it cannot be used as a unique immunotherapeutic target in NS cancer. However, our results do show the immunolocalisation of Sp17 in a proportion of NS tumour cells, but not in their non-pathological counterparts. The emerging complex function of Sp17 makes further studies necessary to clarify the link between it and immunopositive cells.
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影响因子：
--
作者：
Grizzi F;Chiriva-Internati M
通讯作者：
Chiriva-Internati M
影响因子：
5.7
作者：
Debinski, W;Gibo, DM
通讯作者：
Gibo, DM
影响因子：
10.3
作者：
Legler, JM;Ries, LAG;Linet, MS
通讯作者：
Linet, MS
影响因子：
3.2
作者：
Grizzi, F;Chiriva-Internati, M;Kast, WM
通讯作者：
Kast, WM
影响因子：
3.2
作者：
Grizzi, F;Chiriva-Internati, M;Dioguardi, N
通讯作者：
Dioguardi, N