Evolution of nonstop, no-go and nonsense-mediated mRNA decay and their termination factor-derived components.
Evolution of nonstop, no-go and nonsense-mediated mRNA decay and their termination factor-derived components.
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DOI：
10.1186/1471-2148-8-290
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发表时间：
2008-10-23
影响因子：
3.4
通讯作者：
Hauryliuk V
中科院分区：
文献类型：
--
作者：
Atkinson GC;Baldauf SL;Hauryliuk V
真核生物/古细菌特异性eRF1和eRF3蛋白家族成员在翻译终止中起核心作用。它们也是各种mRNA监视机制的核心，包括eRF1平行对话体Dom34p和eRF3平行对话体Hbs1p和Ski7p。利用序列相似性搜索、多序列比对和系统发育分析等方法研究了eRF1和eRF3家族的进化过程。大量BLAST研究证实，Hbs1p和eRF3仅存在于真核生物中，而Dom34p和eRF1 （a/eRF1）则普遍存在于真核生物和古细菌中。Ski7p似乎仅限于酵母属的一个子集。比对表明，Dom34p不具有特征的1类RF微畴GGQ、NIKS和YXCXXXF，这与最近对Dom34p的晶体学分析一致。蛋白质家族的系统发育树使我们能够重建真核生物和古细菌中这些蛋白质介导的mRNA监视机制的进化。我们提出真核生物和古细菌的最后共同祖先具有dom34p介导的no-go衰变（NGD）。这个祖先的Dom34p可能需要也可能不需要trGTPase，主要像a/eEF1A一样，将其传递到核糖体。在真核生物进化的早期阶段，eEF1A被复制，产生eRF3， eRF3被招募用于翻译终止，与eRF1相互作用。eRF3在再次复制之前或之后进化出无义介导的衰变（NMD）活性，产生Hbs1p，我们认为Hbs1p在真核NGD中被招募来辅助eDom34p。最后，子囊菌酵母内的第三个重复产生了Ski7p，它可能专门用于现有Hbs1p在不间断衰变（NSD）中的一个子集。我们认为ski7p介导的NSD可能是一种特殊的机制，可以抵消朊病毒结构域[PSI+]介导的eRF3沉淀引起的停止密码子穿透增加的影响。
Members of the eukaryote/archaea specific eRF1 and eRF3 protein families have central roles in translation termination. They are also central to various mRNA surveillance mechanisms, together with the eRF1 paralogue Dom34p and the eRF3 paralogues Hbs1p and Ski7p. We have examined the evolution of eRF1 and eRF3 families using sequence similarity searching, multiple sequence alignment and phylogenetic analysis. Extensive BLAST searches confirm that Hbs1p and eRF3 are limited to eukaryotes, while Dom34p and eRF1 (a/eRF1) are universal in eukaryotes and archaea. Ski7p appears to be restricted to a subset of Saccharomyces species. Alignments show that Dom34p does not possess the characteristic class-1 RF minidomains GGQ, NIKS and YXCXXXF, in line with recent crystallographic analysis of Dom34p. Phylogenetic trees of the protein families allow us to reconstruct the evolution of mRNA surveillance mechanisms mediated by these proteins in eukaryotes and archaea. We propose that the last common ancestor of eukaryotes and archaea possessed Dom34p-mediated no-go decay (NGD). This ancestral Dom34p may or may not have required a trGTPase, mostly like a/eEF1A, for its delivery to the ribosome. At an early stage in eukaryotic evolution, eEF1A was duplicated, giving rise to eRF3, which was recruited for translation termination, interacting with eRF1. eRF3 evolved nonsense-mediated decay (NMD) activity either before or after it was again duplicated, giving rise to Hbs1p, which we propose was recruited to assist eDom34p in eukaryotic NGD. Finally, a third duplication within ascomycete yeast gave rise to Ski7p, which may have become specialised for a subset of existing Hbs1p functions in non-stop decay (NSD). We suggest Ski7p-mediated NSD may be a specialised mechanism for counteracting the effects of increased stop codon read-through caused by prion-domain [PSI+] mediated eRF3 precipitation.
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影响因子：
64.8
作者：
Amrani, N;Ganesan, R;Jacobson, A
通讯作者：
Jacobson, A
影响因子：
4.5
作者：
Bertram, G;Bell, HA;Stansfield, I
通讯作者：
Stansfield, I
DOI：
10.1073/pnas.91.13.5848
发表时间：
1994-06-21
影响因子：
11.1
作者：
GRENTZMANN, G;BRECHEMIERBAEY, D;BUCKINGHAM, RH
通讯作者：
BUCKINGHAM, RH
影响因子：
1.2
作者：
Hauryliuk, V. V.
通讯作者：
Hauryliuk, V. V.
影响因子：
3.5
作者：
Chapman, B;Brown, C
通讯作者：
Brown, C