In vitro prevention of cataract by Oyster Mushroom Pleurotus florida extract on isolated goat eye lens.
In vitro prevention of cataract by Oyster Mushroom Pleurotus florida extract on isolated goat eye lens.
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牡蛎蘑菇胸膜佛罗里达菌在孤立的山羊眼镜上提取物的体外预防白内障。
DOI：
10.4103/0253-7613.89823
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发表时间：
2011-11
影响因子：
2.4
通讯作者：
Rai G
中科院分区：
文献类型：
--
作者：
Ganeshpurkar A;Bhadoriya SS;Pardhi P;Jain AP;Rai G
本工作的目的是评估佛罗里达提取物对葡萄糖诱导的体外白内障的影响。将山羊眼晶状体分为四组。第I组晶状体在葡萄糖浓度为5.5 mM的人工房水中孵育（正常对照）。将第II组晶状体与浓度为55 mM的葡萄糖（毒性对照）一起孵育。将用葡萄糖浓度55 mM孵育的组III和IV晶状体与250 μg/ml和500 μg/ml的佛罗里达假单胞菌水乙醇提取物一起沿着孵育，并进行形态学和生化评价。II组晶状体显示出高量的丙二醛（MDA）可溶性和不溶性蛋白质，并且降低过氧化氢酶和谷胱甘肽水平，而用佛罗里达提取物处理的晶状体显示出显著（P < 0.05）的MDA降低，过氧化氢酶（P < 0.001）、谷胱甘肽（P < 0.005）以及总蛋白和可溶性蛋白质水平增加。水乙醇提取物的佛罗里达州显示出预防体外葡萄糖诱导的白内障。因此，山羊透镜模型可用于各种抗白内障药物的试验。
The aim of the present work was to evaluate the in vitro effect of Pleurotus florida extract cataract induced by glucose. Goat eye lenses were divided into four groups. Group I lenses were incubated in artificial aqueous humor with glucose concentration 5.5 mM (normal control). Group II lenses were incubated with glucose concentration 55 mM (toxic control). Group III and IV lenses incubated with glucose concentration 55 mM were incubated along with hydroethanolic extract of P. florida 250 μg/ml and 500 μg/ml and subjected to morphological and biochemical evaluation. Group II lenses showed high amount of malondialdehyde (MDA) soluble and insoluble protein and decreased catalase and glutathione levels, while lenses treated with P. florida extract showed significant (P < 0.05) reduction in MDA, increased level of catalase (P < 0.001), glutathione (P < 0.005) and total and soluble protein. Hydroethanolic extract of P. florida showed prevention of in vitro glucose induced cataract. Thus, the goat lens model could be used for testing of various anticataract agents.
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影响因子：
2.6
作者：
Chakraborty, TK;Das, N;Mukherjee, M
通讯作者：
Mukherjee, M
影响因子：
3.4
作者：
Varma, SD;Hegde, KR
通讯作者：
Hegde, KR
影响因子：
5.6
作者：
Christen, WG;Glynn, RJ;Hennekens, CH
通讯作者：
Hennekens, CH
影响因子：
3.3
作者：
Zhao, W;Devamanoharan, PS;Varma, SD
通讯作者：
Varma, SD
影响因子：
3.9
作者：
ELLMAN, GL
通讯作者：
ELLMAN, GL