Enterobacter Strains Might Promote Colon Cancer
Enterobacter Strains Might Promote Colon Cancer
复制标题
肠杆菌菌株可能会促进结肠癌
DOI：
10.1007/s00284-015-0867-x
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发表时间：
2015
影响因子：
2.6
通讯作者：
F. Şahin
中科院分区：
文献类型：
--
作者：
Dilşad Yurdakul;A. Yazgan;F. Şahin
已经进行了许多研究来确定细菌物种和癌症之间的相互作用。然而，尚未尝试证明肠杆菌属之间的可能关系。和结肠癌因此，本研究旨在探讨肠杆菌属菌株对结肠癌的影响。从11种肠杆菌属中分离细菌蛋白，一种摩氏摩根菌和一种大肠杆菌菌株，并应用于NCM 460（Incell）和CRL 1790（ATCC）细胞系。在MTS测定中测定细胞活力和增殖。流式细胞术检测CD24表达及细胞凋亡。进行实时PCR研究以确定NF κ B和Bcl 2表达。使用Graphpad软件进行统计分析。结果表明，从肠杆菌属分离的蛋白质，显著增加了细胞活力和增殖，同时降低了所测试的细胞系的凋亡。本研究中的数据表明肠杆菌属菌株可能促进结肠癌。此外，肠杆菌属（Enterobacter spp.）可能是结肠癌发生和进展的临床重要因素。可以在动物模型上进行研究，以开发新的治疗策略。
Many studies have been performed to determine the interaction between bacterial species and cancer. However, there has been no attempts to demonstrate a possible relationship between Enterobacter spp. and colon cancer so far. Therefore, in the present study, it is aimed to investigate the effects of Enterobacter strains on colon cancer. Bacterial proteins were isolated from 11 Enterobacter spp., one Morganella morganii, and one Escherichia coli strains, and applied onto NCM460 (Incell) and CRL1790 (ATCC) cell lines. Cell viability and proliferation were determined in MTS assay. Flow Cytometry was used to detect CD24 level and apoptosis. Real-Time PCR studies were performed to determine NFKB and Bcl2 expression. Graphpad Software was used for statistical analysis. The results showed that proteins, isolated from the Enterobacter spp., have significantly increased cell viability and proliferation, while decreasing the apoptosis of the cell lines tested. The data in the present study indicated that Enterobacter strains might promote colon cancer. Moreover, Enterobacter spp. could be a clinically important factor for colon cancer initiation and progression. Studies can be extended on animal models in order to develop new strategies for treatment.
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影响因子：
6
作者：
Balish, E;Warner, T
通讯作者：
Warner, T
影响因子：
6.4
作者：
Newman, JV;Kosaka, T;Schauer, DB
通讯作者：
Schauer, DB
影响因子：
158.5
作者：
PARSONNET, J;FRIEDMAN, GD;SIBLEY, RK
通讯作者：
SIBLEY, RK
bcl-2原癌基因与胃肠道上皮肿瘤进展模型。
DOI：
--
发表时间：
1995
期刊：
The American journal of pathology.
影响因子：
--
作者：
Bronner,MP;Culin,C;Reed,JC;Furth,EE
通讯作者：
Furth,EE