Vanin-1 in Renal Pelvic Urine Reflects Kidney Injury in a Rat Model of Hydronephrosis.
Vanin-1 in Renal Pelvic Urine Reflects Kidney Injury in a Rat Model of Hydronephrosis.
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DOI：
10.3390/ijms19103186
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发表时间：
2018-10-16
影响因子：
5.6
通讯作者：
Iwanaga K
中科院分区：
文献类型：
--
作者：
Hosohata K;Jin D;Takai S;Iwanaga K
尿路梗阻和随后肾积水的发展可导致肾损伤，从而导致慢性肾脏疾病。虽然早期发现肾损伤很重要，但新的肾积水生物标志物尚未确定。在这项研究中，我们研究了vanin-1是否可以作为肾积水的潜在生物标志物。对雄性Sprague-Dawley大鼠进行单侧输尿管梗阻（UUO）。UUO后第7天，肾小管组织病理学损伤明显，肾盂尿vanin-1水平显著高于假手术组大鼠的排尿。此外，vanin-1保持在相同的水平，直到第14天。假手术组与UUO组大鼠血清vanin-1水平无显著性差异。肾组织中vanin-1的mRNA和蛋白表达显著降低，而转化生长因子（TGF）-β1和Snail-1的表达增加，Snail-1在肾纤维化的发生中起关键作用。提示肾盂尿中vanin-1是由UUO大鼠肾小管细胞释放的，反映了早期肾小管损伤。尿vanin-1可作为肾积水致肾小管损伤的一个候选生物标志物。
Urinary tract obstruction and the subsequent development of hydronephrosis can cause kidney injuries, which results in chronic kidney disease. Although it is important to detect kidney injuries at an early stage, new biomarkers of hydronephrosis have not been identified. In this study, we examined whether vanin-1 could be a potential biomarker for hydronephrosis. Male Sprague-Dawley rats were subjected to unilateral ureteral obstruction (UUO). On day 7 after UUO, when the histopathological renal tubular injuries became obvious, the vanin-1 level in the renal pelvic urine was significantly higher than that in voided urine from sham-operated rats. Furthermore, vanin-1 remained at the same level until day 14. There was no significant difference in the serum vanin-1 level between sham-operated rats and rats with UUO. In the kidney tissue, the mRNA and protein expressions of vanin-1 significantly decreased, whereas there was increased expression of transforming growth factor (TGF)-β1 and Snail-1, which plays a pivotal role in the pathogenesis of renal fibrosis via epithelial-to-mesenchymal transition (EMT). These results suggest that vanin-1 in the renal pelvic urine is released from the renal tubular cells of UUO rats and reflects renal tubular injuries at an early stage. Urinary vanin-1 may serve as a candidate biomarker of renal tubular injury due to hydronephrosis.
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影响因子：
4.6
作者：
Mei S;Li L;Wei Q;Hao J;Su Y;Mei C;Dong Z
通讯作者：
Dong Z
影响因子：
5.3
作者：
Berruyer, C;Martin, FM;Galland, F
通讯作者：
Galland, F
影响因子：
6
作者：
Rosivatz, E;Becker, I;Becker, KF
通讯作者：
Becker, KF
尿C型利钠肽排泄增加可能是肾小管间质纤维化的早期标志
影响因子：
3
作者：
Hu, Peng;Wang, Jing;Qin, Yuan Han
通讯作者：
Qin, Yuan Han
DOI：
10.1073/pnas.85.24.9557
发表时间：
1988-12-01
影响因子：
11.1
作者：
LISANTI, MP;SARGIACOMO, M;RODRIGUEZBOULAN, E
通讯作者：
RODRIGUEZBOULAN, E