Two deletion variants of Middle East respiratory syndrome coronavirus found in a patient with characteristic symptoms.
Two deletion variants of Middle East respiratory syndrome coronavirus found in a patient with characteristic symptoms.
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在一名有特征症状的患者中发现中东呼吸综合征冠状病毒的两种缺失变种
DOI：
10.1007/s00705-017-3361-x
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发表时间：
2017-08
影响因子：
2.7
通讯作者：
Zhao W
中科院分区：
文献类型：
--
作者：
Xie Q;Cao Y;Su J;Wu J;Wu X;Wan C;He M;Ke C;Zhang B;Zhao W
中东呼吸综合征冠状病毒（MERS CoV）自2012年首次报道以来，从未检测到显着的序列变异。一名MERS患者于2015年5月下旬从韩国入境中国。该患者44岁，出现高烧、干咳、少量痰、气促等症状，与MERS相关症状大致一致，曾与MERS确诊病例有密切接触。经过一个月的抗病毒治疗，经抗感染、免疫增强剂治疗后，患者康复出院。收集鼻咽拭子样本进行直接测序，结果发现 MERS CoV 的两个缺失变体。 ORF5和E蛋白之间发生414和419 nt的缺失，导致ORF5和E蛋白的部分蛋白融合或截短。通过生物信息学进行的功能分析以及与之前研究的比较表明，这两种变体在包装 MERS CoV 的能力方面可能存在缺陷。然而，这些缺失是如何发生的机制以及它们会产生什么影响还需要进一步研究。本文的在线版本 (doi:10.1007/s00705-017-3361-x) 包含补充材料，可供授权用户使用。
Significant sequence variation of Middle East respiratory syndrome coronavirus (MERS CoV) has never been detected since it was first reported in 2012. A MERS patient came from Korea to China in late May 2015. The patient was 44 years old and had symptoms including high fever, dry cough with a little phlegm, and shortness of breath, which are roughly consistent with those associated with MERS, and had had close contact with individuals with confirmed cases of MERS.After one month of therapy with antiviral, anti-infection, and immune-enhancing agents, the patient recovered in the hospital and was discharged. A nasopharyngeal swab sample was collected for direct sequencing, which revealed two deletion variants of MERS CoV. Deletions of 414 and 419 nt occurred between ORF5 and the E protein, resulting in a partial protein fusion or truncation of ORF5 and the E protein. Functional analysis by bioinformatics and comparison to previous studies implied that the two variants might be defective in their ability to package MERS CoV. However, the mechanism of how these deletions occurred and what effects they have need to be further investigated. The online version of this article (doi:10.1007/s00705-017-3361-x) contains supplementary material, which is available to authorized users.
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影响因子：
3.7
作者：
Nieto-Torres JL;Dediego ML;Alvarez E;Jiménez-Guardeño JM;Regla-Nava JA;Llorente M;Kremer L;Shuo S;Enjuanes L
通讯作者：
Enjuanes L
影响因子：
5.4
作者：
Du, Lanying;Zhao, Guangyu;Jiang, Shibo
通讯作者：
Jiang, Shibo
影响因子：
5.4
作者：
Gao, Jing;Lu, Guangwen;Gao, George F.
通讯作者：
Gao, George F.
影响因子：
5.8
作者：
Möller, S;Croning, MDR;Apweiler, R
通讯作者：
Apweiler, R
影响因子：
5.4
作者：
VANMARLE, G;LUYTJES, W;SPAAN, WJM
通讯作者：
SPAAN, WJM