Molecular surveillance of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi by genotyping and subtyping parasites in wastewater.
Molecular surveillance of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi by genotyping and subtyping parasites in wastewater.
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通过废水中寄生虫的基因分型和分型对隐孢子虫属、十二指肠贾第鞭毛虫和比氏肠细胞虫进行分子监测
DOI：
10.1371/journal.pntd.0001809
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发表时间：
2012
影响因子：
3.8
通讯作者：
Feng Y
中科院分区：
文献类型：
--
作者：
Li N;Xiao L;Wang L;Zhao S;Zhao X;Duan L;Guo M;Liu L;Feng Y
尽管隐孢子虫病和贾第虫病广泛发生，但世界卫生组织认为它们是被忽视的疾病。人们对发展中国家人类中这些疾病和微孢子虫病的流行病学知之甚少。未经处理的污水中病原体的浓度很高，这使得通过对少量样本进行基因型和亚型分析来表征这些病原体的传播变得简单。隐孢子虫基因型和亚型的分布，采用PCR-测序技术对上海、南京、武汉和青岛市386份合流制污水管样中的贾第鞭毛虫、贾第鞭毛虫和比氏肠胞虫进行了检测。艾美耳球虫属也进行基因分型以评估家畜对隐孢子虫属的贡献，G. sporadalis和E. bieneusi在废水中。隐孢子虫的高发生率。(56.2%）、G.红腹叶蝉（82.6%）、E. bieneusi（87.6%）和Eimeria/Cyclospora（80.3%）的来源。正如预期的那样，几种人类致病性物种/基因型，包括隐孢子虫hominis，隐孢子虫gragridis，G。A-II亚组合和E. D基因型为优势寄生虫。除了人类，隐孢子虫属的常见存在。和艾美耳球虫属（Eimeria spp.）表明啮齿类动物可能是引起E. bieneusi基因型D。同样，艾美耳球虫属的发现。和鸟类隐孢子虫C. meleagridis might be of both human and bird origins.隐孢子虫属隐孢子虫G. Escherialis基因型和亚型，以及E.城市污水中的bieneusi基因型表明，人传人似乎是重要的流行病学隐孢子虫病，贾第鞭毛虫病，微孢子虫病在研究领域。上海和武汉两地不同亚型分布的发现，提示了C.中国不同地区的人。
Despite their wide occurrence, cryptosporidiosis and giardiasis are considered neglected diseases by the World Health Organization. The epidemiology of these diseases and microsporidiosis in humans in developing countries is poorly understood. The high concentration of pathogens in raw sewage makes the characterization of the transmission of these pathogens simple through the genotype and subtype analysis of a small number of samples. The distribution of genotypes and subtypes of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in 386 samples of combined sewer systems from Shanghai, Nanjing and Wuhan and the sewer system in Qingdao in China was determined using PCR-sequencing tools. Eimeria spp. were also genotyped to assess the contribution of domestic animals to Cryptosporidium spp., G. duodenalis, and E. bieneusi in wastewater. The high occurrence of Cryptosporidium spp. (56.2%), G. duodenalis (82.6%), E. bieneusi (87.6%), and Eimeria/Cyclospora (80.3%) made the source attribution possible. As expected, several human-pathogenic species/genotypes, including Cryptosporidium hominis, Cryptosporidium meleagridis, G. duodenalis sub-assemblage A-II, and E. bieneusi genotype D, were the dominant parasites in wastewater. In addition to humans, the common presence of Cryptosporidium spp. and Eimeria spp. from rodents indicated that rodents might have contributed to the occurrence of E. bieneusi genotype D in samples. Likewise, the finding of Eimeria spp. and Cryptosporidium baileyi from birds indicated that C. meleagridis might be of both human and bird origins. The distribution of Cryptosporidium species, G. duodenalis genotypes and subtypes, and E. bieneusi genotypes in urban wastewater indicates that anthroponotic transmission appeared to be important in epidemiology of cryptosporidiosis, giardiasis, and microsporidiosis in the study areas. The finding of different distributions of subtypes between Shanghai and Wuhan was indicative of possible differences in the source of C. hominis among different areas in China.
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影响因子：
11.8
作者：
Feng Y;Wang L;Duan L;Gomez-Puerta LA;Zhang L;Zhao X;Hu J;Zhang N;Xiao L
通讯作者：
Xiao L
影响因子：
2.6
作者：
Talebi, Malihe;Rahimi, Fateh;Pourshafie, Mohammad R.
通讯作者：
Pourshafie, Mohammad R.
影响因子：
4
作者：
Robinson, Guy;Wright, Steve;Chalmers, Rachel M.
通讯作者：
Chalmers, Rachel M.
影响因子：
2
作者：
Feng, Yaoyu;Ortega, Ynes;Xiao, Lihua
通讯作者：
Xiao, Lihua
影响因子：
2.7
作者：
Carducci, A.;Verani, M.;Casini, B.
通讯作者：
Casini, B.