Comparative genomics of the Western Hemisphere soft tick-borne relapsing fever borreliae highlights extensive plasmid diversity.
Comparative genomics of the Western Hemisphere soft tick-borne relapsing fever borreliae highlights extensive plasmid diversity.
复制标题
DOI：
10.1186/s12864-022-08523-7
复制
发表时间：
2022-05-31
期刊：
影响因子：
4.4
通讯作者：
中科院分区：
文献类型：
--
作者：
蜱传回归热 (TBRF) 是一种全球流行但尚未得到充分研究的媒介传播疾病，由软体和硬体蜱传播。虽然软 TBRF (sTBRF) 螺旋体的描述已经有一个多世纪了，但我们对促进载体和宿主适应的分子机制知之甚少。这是因为它们的基因组虽小（~ 1.5 Mb）但片段化，通常由线性染色体以及线性和环状质粒组成，非常复杂。大多数 sTBRF 螺旋体基因组的质粒序列要么缺失，要么以未组装的序列形式存放。因此，我们的目标是生成完整的、质粒解析的基因组，用于对西半球 sTBRF 物种进行比较分析。利用伯氏疏螺旋体特异性管道，结合短读长和长读长测序技术，对西半球 sTBRF 螺旋体的基因组进行了测序和组装。分析中包括最近从中美洲和南美洲分离出的两个物种，Borrelia puertoricensis n. sp。和委内瑞拉疏螺旋体。质粒分析鉴定出不同的序列，将质粒分为 30 个家族；然而，只有三个科在所有物种中都是保守且同线性的。我们还比较了两个物种，B. venezuelensis 和 Borrelia turicatae，这两个物种相距 6,800 公里，来自不同的蜱虫媒介物种，但之前报道它们在遗传上相似。为了真正了解 TBRF 螺旋体物种之间观察到的生物学差异，需要完整的染色体和质粒序列。这种比较基因组分析强调了跨物种的高染色体同线性和多样化的质粒组成。对于 B. turicatae 和 B. venezuelensis 来说尤其如此，它们具有较高的平均核苷酸同一性和广泛的质粒多样性。这些发现为未来评估质粒在载体和宿主适应中的作用奠定了基础。在线版本包含可在 10.1186/s12864-022-08523-7 获取的补充材料。
Tick-borne relapsing fever (TBRF) is a globally prevalent, yet under-studied vector-borne disease transmitted by soft and hard bodied ticks. While soft TBRF (sTBRF) spirochetes have been described for over a century, our understanding of the molecular mechanisms facilitating vector and host adaptation is poorly understood. This is due to the complexity of their small (~ 1.5 Mb) but fragmented genomes that typically consist of a linear chromosome and both linear and circular plasmids. A majority of sTBRF spirochete genomes’ plasmid sequences are either missing or are deposited as unassembled sequences. Consequently, our goal was to generate complete, plasmid-resolved genomes for a comparative analysis of sTBRF species of the Western Hemisphere. Utilizing a Borrelia specific pipeline, genomes of sTBRF spirochetes from the Western Hemisphere were sequenced and assembled using a combination of short- and long-read sequencing technologies. Included in the analysis were the two recently isolated species from Central and South America, Borrelia puertoricensis n. sp. and Borrelia venezuelensis, respectively. Plasmid analyses identified diverse sequences that clustered plasmids into 30 families; however, only three families were conserved and syntenic across all species. We also compared two species, B. venezuelensis and Borrelia turicatae, which were isolated ~ 6,800 km apart and from different tick vector species but were previously reported to be genetically similar. To truly understand the biological differences observed between species of TBRF spirochetes, complete chromosome and plasmid sequences are needed. This comparative genomic analysis highlights high chromosomal synteny across the species yet diverse plasmid composition. This was particularly true for B. turicatae and B. venezuelensis, which had high average nucleotide identity yet extensive plasmid diversity. These findings are foundational for future endeavors to evaluate the role of plasmids in vector and host adaptation. The online version contains supplementary material available at 10.1186/s12864-022-08523-7.
登录
查看更多内容
DOI：
10.1016/0035-9203(90)90106-o
发表时间：
1990-11-01
影响因子：
2.2
作者：
BARCLAY, AJG;COULTER, JBS
通讯作者：
COULTER, JBS
影响因子：
3.6
作者：
Casjens, S;Murphy, M;Huang, WM
通讯作者：
Huang, WM
影响因子：
2.4
作者：
Campbell, Stefanie B.;Klioueva, Anna;Hinckley, Alison
通讯作者：
Hinckley, Alison
影响因子：
4.6
作者：
Binetruy, Florian;Garnier, Stephane;Duron, Olivier
通讯作者：
Duron, Olivier
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.