Expression of Cytokeratins 13 and 16 in Middle Ear Cholesteatoma
Expression of Cytokeratins 13 and 16 in Middle Ear Cholesteatoma
复制标题
细胞角蛋白13和16在中耳胆脂瘤中的表达
DOI：
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
C. Huang
中科院分区：
文献类型：
--
作者：
H. Sasaki;C. Huang
中耳腔角化上皮的堆积是胆脂瘤发病的重要因素。我们推测，耳道皮肤的角质形成细胞因中耳腔的炎症而迁移和过度肥大，从而导致角蛋白碎片的积累。在本研究中，我们研究了特异性细胞角蛋白(CKs)在胆脂瘤基质中的表达，以确定胆脂瘤是否是一种增生性疾病。用两种单抗检测胆脂瘤、耳道皮肤和鼓膜中细胞角蛋白的表达，其中一种是同时表达细胞角蛋白13和16(抗体K8.12)，另一种是仅表达细胞角蛋白13(抗体K5-1A3)。CK 13(相对分子质量51kD)是角质形成细胞分化的标志，CK 16(相对分子质量48kD)是角质形成细胞过度增殖的标志。免疫印迹探针的使用表明，胆脂瘤中存在CK13和CK16。免疫荧光染色显示CK16位于胆脂瘤的基底层上，位于外耳道附近。CK16也定位于鼻道皮肤和鼓膜的基底层。CK13定位于胆脂瘤的基底层，位于外耳道的远侧，但在耳道皮肤和鼓膜中未见表达。综上所述，本资料提示胆脂瘤是一种过度增生性疾病，胆脂瘤在外耳道附近表达CK16，并在生长过程中向远端转化为CK13。
The accumulation of keratinizing epithelium in the middle ear cavity is a crucial factor in the pathogenesis of cholesteatoma. We hypothesize that keratinocytes from the skin of the ear canal migrate and hyperprollferate in response to Inflammation in the middle ear cavity to cause accumulation of keratin debris. In the present study, we Investigated the expression of specific cytokeratins (CKs) in the cholesteatoma matrix to determine whether cholesteatoma is a hyperproliferative disease. Cytokeratin expression was examined in cholesteatoma, meatal skin, and tympanic membrane with two monoclonal antibodies, one for both cytokeratins 13 and 16 (antibody K8.12), and another for cytokeratin 13 only (antibody K5–1A3). CK 13 (MW 51 KD) Is a marker of differentiation and CK 16 (MW 48 KD) is a marker of hyperproliferatlon of keratinocytes. The use of immunoblot probes showed that CKs 13 and 16 were present in cholesteatoma. Immunofluorescenf staining showed the presence of CK 16 In the suprabasal layer of cholesteatoma, which was located near the external ear canal. CK 16 was also localized in the suprabasal layer of meatal skin and tympanic membrane. CK 13 was localized in the basal layer of the cholesteatoma, distal to the external ear canal, but not in the meatal skin and tympanic membrane. Taken together, the present data suggest that cholesteatoma is a hyperprollferative disease and that cholesteatoma expresses CK 16 near the external ear canal and transforms to express CK 13 during growth distally.
角蛋白在深部浸润性基底细胞癌和鳞状细胞癌中的表达：一项免疫组织化学研究。
DOI：
10.1111/j.1524-4725.1987.tb03951.x
发表时间：
1987
期刊：
The Journal of dermatologic surgery and oncology
影响因子：
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作者：
Robinson,JK
通讯作者：
Robinson,JK
DOI：
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发表时间：
1985-03
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
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作者：
D. Cooper;A. Schermer;T. Sun
通讯作者：
D. Cooper;A. Schermer;T. Sun
影响因子：
11.2
作者：
Michael P. Grace;Kwan Hee Kim;Lawrence D. True;Elaine Fuchs
通讯作者：
Michael P. Grace;Kwan Hee Kim;Lawrence D. True;Elaine Fuchs