Competitive growth experiments with a high-lipid Chlamydomonas reinhardtii mutant strain and its wild-type to predict industrial and ecological risks.
Competitive growth experiments with a high-lipid Chlamydomonas reinhardtii mutant strain and its wild-type to predict industrial and ecological risks.
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DOI：
10.1186/s13568-016-0305-x
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发表时间：
2017-12
期刊：
影响因子：
3.7
通讯作者：
Pandhal J
中科院分区：
文献类型：
--
作者：
Russo DA;Beckerman AP;Pandhal J
目前正在利用大规模养殖系统将关键微藻物种作为生物制造平台进行开发。随着基因操作和代谢工程工具的迅速发展，提高生产率水平或生产非本地化合物的机会正在增加。无论最终产品是什么，露天池塘养殖突变微藻菌株都存在环境和工业风险。突变的逃逸可能会损害当地的生物多样性，并增加藻类大量繁殖的风险。同样，如果养殖池受到野生型(WT)微藻的入侵，或者突变株恢复到WT表型，生产力可能会受到影响。为了研究这些潜在的风险，应用响应面方法确定了两株衣藻的竞争结果，一株是WT(CC-124)，一株是高脂积累突变体(CC-4333)，它们生长在不同氮素水平和初始WT与突变体比例的混合营养条件下。生长实验结果表明，突变细胞的生长速度是单一培养的WT细胞的指数生长速度的两倍。然而，由于从滞后期到指数期的转换较慢，突变细胞在每一次共培养处理中都被WT击败。这表明，在测试的条件下，室外培养的莱茵梭菌细胞壁缺陷突变株在逃生情况下不会对其WT带来重大的环境风险。此外，脂类结果显示，与WT相比，突变菌株在50 mg L−1NH4Cl中每个细胞积累的标签增加了200%以上，因此，突变菌株的脆弱性可能会影响整体工业生产率。本文的在线版本(doi：10.1186/s13568-0160305-x)包含补充材料，授权用户可以使用。
Key microalgal species are currently being exploited as biomanufacturing platforms using mass cultivation systems. The opportunities to enhance productivity levels or produce non-native compounds are increasing as genetic manipulation and metabolic engineering tools are rapidly advancing. Regardless of the end product, there are both environmental and industrial risks associated to open pond cultivation of mutant microalgal strains. A mutant escape could be detrimental to local biodiversity and increase the risk of algal blooms. Similarly, if the cultivation pond is invaded by a wild-type (WT) microalgae or the mutant reverts to WT phenotypes, productivity could be impacted. To investigate these potential risks, a response surface methodology was applied to determine the competitive outcome of two Chlamydomonas reinhardtii strains, a WT (CC-124) and a high-lipid accumulating mutant (CC-4333), grown in mixotrophic conditions, with differing levels of nitrogen and initial WT to mutant ratios. Results of the growth experiments show that mutant cells have double the exponential growth rate of the WT in monoculture. However, due to a slower transition from lag phase to exponential phase, mutant cells are outcompeted by the WT in every co-culture treatment. This suggests that, under the conditions tested, outdoor cultivation of the C. reinhardtii cell wall-deficient mutant strains does not carry a significant environmental risk to its WT in an escape scenario. Furthermore, lipid results show the mutant strain accumulates over 200% more TAGs per cell, at 50 mg L−1 NH4Cl, compared to the WT, therefore, the fragility of the mutant strain could impact on overall industrial productivity. The online version of this article (doi:10.1186/s13568-016-0305-x) contains supplementary material, which is available to authorized users.
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