Modern Solutions for Ancient Pathogens: Direct Pathogen Sequencing for Diagnosis of Lepromatous Leprosy and Cerebral Coenurosis.

Modern Solutions for Ancient Pathogens: Direct Pathogen Sequencing for Diagnosis of Lepromatous Leprosy and Cerebral Coenurosis.
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应对古老病原体的现代解决方案:直接病原体测序用于瘤型麻风及脑多头蚴病的诊断

DOI:
10.1093/ofid/ofac428
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发表时间:
2022-09
影响因子:
4.2
通讯作者:
--
中科院分区:
医学3区
文献类型:
--
作者:

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无法在体外培养的微生物在诊断上仍然具有挑战性,历史上依赖于临床发现,组织学或靶向分子检测。我们应用全基因组测序直接从组织诊断感染分枝杆菌(麻风病)和寄生虫(多头蚴病)。直接病原体DNA测序提供了灵活的解决方案,诊断困难的病原体在不同的情况下。
Microbes unculturable in vitro remain diagnostically challenging, dependent historically on clinical findings, histology, or targeted molecular detection. We applied whole-genome sequencing directly from tissue to diagnose infections with mycobacteria (leprosy) and parasites (coenurosis). Direct pathogen DNA sequencing provides flexible solutions to diagnosis of difficult pathogens in diverse contexts.
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