Genome-Wide Investigation of the NAC Transcription Factor Family in Miscanthus sinensis and Expression Analysis Under Various Abiotic Stress.
Genome-Wide Investigation of the NAC Transcription Factor Family in Miscanthus sinensis and Expression Analysis Under Various Abiotic Stress.
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芒属植物NAC转录因子家族的全基因组研究及其在不同非生物胁迫下的表达分析
DOI：
10.3389/fpls.2021.766550
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发表时间：
2021
影响因子：
5.6
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Nie G;Yang Z;He J;Liu A;Chen J;Wang S;Wang X;Feng G;Li D;Peng Y;Huang L;Zhang X
NAC转录因子家族被认为是植物特有的大型基因家族，在植物发育和逆境响应中发挥重要作用。芒草通常作为饲料、观赏草或生物能源作物种植在大片边缘地区，对非生物胁迫的抵抗力要求相对较高。最近发布的中华木犀染色体级组装基因组草案为NAC蛋白的全基因组研究提供了基础平台。本研究共鉴定了261个中华木犀NAC基因，并对该基因家族进行了完整的概述，包括基因结构、保守基序组成、染色体分布和基因重复。结果表明，NAC家族的基因长度、分子量（MW）和理论等电点（pI）存在差异，而基因结构和基序相对保守。染色体作图分析发现中华贻贝NAC基因在19条中华贻贝染色体上分布不均匀，染色体间进化分析发现9对串联重复基因和121个节段重复，提示基因重复，尤其是节段重复可能与中华贻贝NAC基因家族的扩增有关。分析了中华海棠SNAC亚群14个基因在高盐、PEG和重金属条件下的表达模式，发现多个NAC基因可以通过处理被诱导。这些结果将为后续研究NAC基因在中华木鳖胁迫反应机制中的功能特征及其在发育中的潜在作用提供非常有用的参考。
The NAC transcription factor family is deemed to be a large plant-specific gene family that plays important roles in plant development and stress response. Miscanthus sinensis is commonly planted in vast areas of marginal lang as forage, ornamental grass, or bioenergy crop which demand a relatively high resistance to abiotic stresses. The recent release of a draft chromosome-scale assembly genome of M. sinensis provided a basic platform for the genome-wide investigation of NAC proteins. In this study, a total of 261 M. sinensis NAC genes were identified and a complete overview of the gene family was presented, including gene structure, conserved motif compositions, chromosomal distribution, and gene duplications. Results showed that gene length, molecular weights (MW), and theoretical isoelectric points (pI) of NAC family were varied, while gene structure and motifs were relatively conserved. Chromosomal mapping analysis found that the M. sinensis NAC genes were unevenly distributed on 19 M. sinensis chromosomes, and the interchromosomal evolutionary analysis showed that nine pairs of tandem duplicate genes and 121 segmental duplications were identified, suggesting that gene duplication, especially segmental duplication, is possibly associated with the amplification of M. sinensis NAC gene family. The expression patterns of 14 genes from M. sinensis SNAC subgroup were analyzed under high salinity, PEG, and heavy metals, and multiple NAC genes could be induced by the treatment. These results will provide a very useful reference for follow-up study of the functional characteristics of NAC genes in the mechanism of stress-response and potential roles in the development of M. sinensis.
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影响因子：
7
作者：
Blanc, G;Hokamp, K;Wolfe, KH
通讯作者：
Wolfe, KH
影响因子：
4.4
作者：
Kim, Sang-Gyu;Lee, Sangmin;Park, Chung-Mo
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影响因子：
5.1
作者：
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影响因子：
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作者：
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通讯作者：
Noble WS
影响因子：
3
作者：
Golfier, Philippe;Volkert, Christopher;Wolf, Sebastian
通讯作者：
Wolf, Sebastian