Controllable microfluidic synthesis of multiphase drug-carrying lipospheres for site-targeted therapy.
Controllable microfluidic synthesis of multiphase drug-carrying lipospheres for site-targeted therapy.
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DOI：
10.1002/btpr.214
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发表时间：
2009-07
影响因子：
2.9
通讯作者：
Dayton, Paul A.
中科院分区：
文献类型：
--
作者：
Hettiarachchi, Kanaka;Lee, Abraham P.;Zhang, Shirley;Feingold, Steven;Dayton, Paul A.
我们报道了利用数字微流控技术生产微米级充气脂球用于化疗药物输送。芯片上合成的优点包括单分散的粒度分布(多分散指数(σ)为<；5%)，具有稳定的稳定性和均匀的载药量。应用光刻技术制备了基于PDMS的新型微流控器件，该器件具有组合的双流体动力聚焦区域和窄孔扩张的喷嘴几何形状。球形载体是通过流动聚焦形成的，磷脂自组装到气体核心周围的脂层，然后在孔口发生剪切诱导的破裂。在外部脂质外壳和内部气泡气核之间包裹额外的油层，允许在高浓度下安全地运输高度疏水和有毒的药物。阿霉素(DOX)的包封率估计为15毫克毫升−1，颗粒堆积在一个有序的单层中。此外，靶向配体附着在脂质外壳上，允许直接与癌细胞结合。对这些单分散气体脂球的初步声学研究表明，高度均匀的回声相关性大于95%。存在利用超声能量进行局部药物浓缩和释放的可能性。
We report the production of micrometer-sized gas-filled lipospheres using digital microfluidics technology for chemotherapeutic drug delivery. Advantages of on-chip synthesis include a monodisperse size distribution (polydispersity index (σ) values of <5%) with consistent stability and uniform drug loading. Photolithography techniques are applied to fabricate novel PDMS-based microfluidic devices that feature a combined dual hydrodynamic flow-focusing region and expanding nozzle geometry with a narrow orifice. Spherical vehicles are formed through flow-focusing by the self-assembly of phospholipids to a lipid layer around the gas core, followed by a shear-induced break off at the orifice. The encapsulation of an extra oil layer between the outer lipid shell and inner bubble gaseous core allows the safe transport of highly hydrophobic and toxic drugs at high concentrations. Doxorubicin (Dox) entrapment is estimated at 15 mg mL−1 of particles packed in a single ordered layer. In addition, the attachment of targeting ligands to the lipid shell allows for direct vehicle binding to cancer cells. Preliminary acoustic studies of these monodisperse gas lipospheres reveal a highly uniform echo correlation of greater than 95%. The potential exists for localized drug concentration and release with ultrasound energy.
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影响因子：
3.5
作者：
Chomas, JE;Dayton, P;Ferrara, K
通讯作者：
Ferrara, K
影响因子：
37.8
作者：
Ellegala, DB;Poi, HL;Lindner, JR
通讯作者：
Lindner, JR
影响因子：
4.4
作者：
Gabizon, Alberto A.;Shmeeda, Hilary;Zalipsky, Samuel
通讯作者：
Zalipsky, Samuel
影响因子：
2.3
作者：
FORSBERG, F;LIU, JB;GOLDBERG, BB
通讯作者：
GOLDBERG, BB
影响因子：
4.6
作者：
Gordillo, JM;Cheng, ZD;Weitz, DA
通讯作者：
Weitz, DA