Permeability assessment of the focused ultrasound-induced blood-brain barrier opening using dynamic contrast-enhanced MRI.
Permeability assessment of the focused ultrasound-induced blood-brain barrier opening using dynamic contrast-enhanced MRI.
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DOI：
10.1088/0031-9155/55/18/012
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发表时间：
2010-09-21
影响因子：
3.5
通讯作者：
Konofagou EE
中科院分区：
文献类型：
--
作者：
Vlachos F;Tung YS;Konofagou EE
聚焦超声（FUS）结合微泡已被证明可以成功地打开小鼠大脑中的血脑屏障（BBB）。在这项研究中，我们计算了血脑屏障通透性开放后，在体内。使用动态对比增强MRI（DCE-MRI）评估BBB开放区域的空间通透性。使用一般动力学模型（GKM）和参考区域模型（RRM）对DCE-MR图像进行后处理。生成渗透性图，并计算每只小鼠超声处理和对照区域中预定义目标体积的Ktranss值。结果表明，BBB开放区的Ktranss（GKM为0.02 ± 0.0123，RRM为0.03 ± 0.0167 min-1）比对侧（对照）（分别为0和8.5 × 10 - 4 ± 12 × 10 - 4 min-1）至少高两个数量级。用两种模型获得的渗透率值显示出统计学显著的一致性和良好的相关性（R2 = 0.97）。在组织学检查时，得出的结论是未诱导肉眼可见的损伤。因此，本研究构成了使用DCE-MRI对FUS诱导的BBB开放的首次渗透性评估，支持了上述技术可能构成安全、非侵入性和有效的药物递送方法的事实。
Focused ultrasound (FUS) in conjunction with microbubbles has been shown to successfully open the blood–brain barrier (BBB) in the mouse brain. In this study, we compute the BBB permeability after opening in vivo. The spatial permeability of the BBB-opened region was assessed using dynamic contrast-enhanced MRI (DCE-MRI). The DCE-MR images were post-processed using the general kinetic model (GKM) and the reference region model (RRM). Permeability maps were generated and the Ktrans values were calculated for a predefined volume of interest in the sonicated and the control area for each mouse. The results demonstrated that Ktrans in the BBB-opened region (0.02 ± 0.0123 for GKM and 0.03 ± 0.0167 min−1 for RRM) was at least two orders of magnitude higher when compared to the contra-lateral (control) side (0 and 8.5 × 10−4 ± 12 × 10−4 min−1, respectively). The permeability values obtained with the two models showed statistically significant agreement and excellent correlation (R2 = 0.97). At histological examination, it was concluded that no macroscopic damage was induced. This study thus constitutes the first permeability assessment of FUS-induced BBB opening using DCE-MRI, supporting the fact that the aforementioned technique may constitute a safe, non-invasive and efficacious drug delivery method.
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影响因子：
19.7
作者：
Hynynen, K;McDannold, N;Jolesz, FA
通讯作者：
Jolesz, FA
DOI：
10.1109/tbme.2009.2034533
发表时间：
2010-01
期刊：
IEEE transactions on bio-medical engineering
影响因子：
--
作者：
Choi JJ;Feshitan JA;Baseri B;Wang S;Tung YS;Borden MA;Konofagou EE
通讯作者：
Konofagou EE
DOI：
10.1073/pnas.0901807106
发表时间：
2009-04-21
影响因子：
11.1
作者：
Li, Lin Z.;Zhou, Rong;Glickson, Jerry D.
通讯作者：
Glickson, Jerry D.
影响因子：
2.9
作者：
Brown, RC;Egleton, RD;Davis, TP
通讯作者：
Davis, TP
影响因子：
2.9
作者：
Hsiao, Ing-Tsung;Liao, Yen-Peng;Liu, Ho-Ling
通讯作者：
Liu, Ho-Ling