Ultra-stable organic fluorophores for single-molecule research.
Ultra-stable organic fluorophores for single-molecule research.
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DOI：
10.1039/c3cs60237k
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发表时间：
2014-02-21
影响因子：
46.2
通讯作者：
Blanchard SC
中科院分区：
文献类型：
--
作者：
Zheng Q;Juette MF;Jockusch S;Wasserman MR;Zhou Z;Altman RB;Blanchard SC
荧光提供了在生物成像中实现对比度的机制，其使得能够以高空间和时间分辨率研究分子结构、动力学和功能。小分子有机荧光团已被证明对这些努力至关重要，并广泛用于单分子和超分辨率显微镜等先进应用。然而，有机荧光团，像所有的荧光物种，表现出不稳定的发射特性，包括闪烁和光漂白，限制了他们的效用和性能。在这里，我们回顾了有机荧光团的光物理和光化学，因为它们涉及到减轻这种不稳定性，通过衍生化稳定的荧光团的发展的具体重点。自愈有机荧光团，其中三重态被共价连接的保护剂分子内猝灭，表现出显着改善的光稳定性。我们讨论了进一步增强的潜力，以实现开发跨越可见光谱的“超稳定”荧光团的目标，以及这些荧光团可能如何影响单分子研究的未来。
Fluorescence provides a mechanism for achieving contrast in biological imaging that enables investigations of molecular structure, dynamics, and function at high spatial and temporal resolution. Small-molecule organic fluorophores have proven essential for such efforts and are widely used in advanced applications such as single-molecule and super-resolution microscopy. Yet, organic fluorophores, like all fluorescent species, exhibit instabilities in their emission characteristics, including blinking and photobleaching that limit their utility and performance. Here, we review the photophysics and photochemistry of organic fluorophores as they pertain to mitigating such instabilities, with a specific focus on the development of stabilized fluorophores through derivatization. Self-healing organic fluorophores, wherein the triplet state is intramolecularly quenched by a covalently attached protective agent, exhibit markedly improved photostabilities. We discuss the potential for further enhancements towards the goal of developing “ultra-stable” fluorophores spanning the visible spectrum and how such fluorophores are likely to impact the future of single-molecule research.
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影响因子：
48
作者：
Biteen, Julie S.;Thompson, Michael A.;Tselentis, Nicole K.;Bowman, Grant R.;Shapiro, Lucy;Moerner, W. E.
通讯作者：
Moerner, W. E.
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通讯作者：
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