Quantum dots find their stride in single molecule tracking.

Quantum dots find their stride in single molecule tracking.
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DOI:
10.1016/j.cbpa.2011.10.011
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发表时间:
2011-12
影响因子:
7.8
通讯作者:
Bruchez, Marcel P.
Bruchez, Marcel P.
中科院分区:
生物学2区
文献类型:
--
作者:
Bruchez, Marcel P.

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量子点作为生物成像剂的演示后十三年,以及在生物偶联的量子点的初始商业引入后的九年,量子点的亮度和光稳定性已使单个分子跟踪的单个材料进行了多种多样的单个材料,从而使单个材料的动力学范围尤其是在单个分子跟踪中。在较短的暴露时间和高指向精度的长时间尺度上,新的方法正在出现,其中量子点用作量子内隔室的“硬球”问题。
Thirteen years after the demonstration of quantum dots as biological imaging agents, and nine years after the initial commercial introduction of bioconjugated quantum dots, the brightness and photostability of the quantum dots has enabled a range of investigations using single molecule tracking. These materials are being routinely utilized by a number of groups to track the dynamics of single molecules in reconstituted biophysical systems and on living cells, and are especially powerful for investigations of single molecules over long timescales with short exposure times and high pointing accuracy. New approaches are emerging where the quantum dots are used as “hard-sphere” probes for intracellular compartments. Innovations in quantum dot surface modification are poised to substantially expand the utility of these materials.
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