Intrinsic terminators in Mycoplasma hyopneumoniae transcription.
Intrinsic terminators in Mycoplasma hyopneumoniae transcription.
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DOI：
10.1186/s12864-015-1468-6
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发表时间：
2015-04-08
期刊：
影响因子：
4.4
通讯作者：
Schrank IS
中科院分区：
文献类型：
--
作者：
Fritsch TE;Siqueira FM;Schrank IS
猪支原体是一种重要的猪源性病原菌，其基因组鸟嘌呤和胞嘧啶（GC）含量低。M.猪链球菌基因组以长的转录单位组织，启动子序列已被定位在所有转录单位的上游。这些分析提供了深入了解基因组规模的基因组织和转录起始。然而，转录终止子序列在M.对猪链球菌基因组了解甚少。计算机模拟分析表明，考虑到不同类型的终止子，在33个单顺反子单元（mCs）的82%和116个多顺反子单元（pCs）的74%中存在推定的终止子。通过RT-PCR和qPCR证实了23个内在终止子的功能活性。通过三种软件算法发现的所有终止子的分析，结合实验结果，使我们能够提出终止过程中RNA发夹形成的模式，并预测终止子在M.猪链球菌基因组序列。由于鸟嘌呤和胞嘧啶含量低，支原体固有终止子的茎环结构与其他细菌中发现的终止子模式不同。In M.在低聚物中，转录可以在转录单位之后和其终止子序列之前结束，并且也可以继续通过终止子序列，其中RNA聚合酶逐渐释放RNA。本文的在线版本（doi：10.1186/s12864-015-1468-6）包含补充材料，可供授权用户使用。
Mycoplasma hyopneumoniae, an important pathogen of swine, exhibits a low guanine and cytosine (GC) content genome. M. hyopneumoniae genome is organised in long transcriptional units and promoter sequences have been mapped upstream of all transcription units. These analysis provided insights into the gene organisation and transcription initiation at the genome scale. However, the presence of transcriptional terminator sequences in the M. hyopneumoniae genome is poorly understood. In silico analyses demonstrated the presence of putative terminators in 82% of the 33 monocistronic units (mCs) and in 74% of the 116 polycistronic units (pCs) considering different classes of terminators. The functional activity of 23 intrinsic terminators was confirmed by RT-PCR and qPCR. Analysis of all terminators found by three software algorithms, combined with experimental results, allowed us to propose a pattern of RNA hairpin formation during the termination process and to predict the location of terminators in the M. hyopneumoniae genome sequence. The stem-loop structures of intrinsic terminators of mycoplasma diverge from the pattern of terminators found in other bacteria due the low content of guanine and cytosine. In M. hyopneumoniae, transcription can end after a transcriptional unit and before its terminator sequence and can also continue past the terminator sequence with RNA polymerases gradually releasing the RNA. The online version of this article (doi:10.1186/s12864-015-1468-6) contains supplementary material, which is available to authorized users.
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影响因子：
3.7
作者：
Siqueira FM;Gerber AL;Guedes RL;Almeida LG;Schrank IS;Vasconcelos AT;Zaha A
通讯作者：
Zaha A
DOI：
10.1093/dnares/dsr028
发表时间：
2011-12
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
--
作者：
Siqueira FM;Schrank A;Schrank IS
通讯作者：
Schrank IS
影响因子：
4.2
作者：
Arrebola E;Carrión VJ;Cazorla FM;Pérez-García A;Murillo J;de Vicente A
通讯作者：
de Vicente A
影响因子：
4.4
作者：
Siqueira FM;Thompson CE;Virginio VG;Gonchoroski T;Reolon L;Almeida LG;da Fonsêca MM;de Souza R;Prosdocimi F;Schrank IS;Ferreira HB;de Vasconcelos AT;Zaha A
通讯作者：
Zaha A
枯草芽孢杆菌和相关物种中转录终止剂的预测。
影响因子：
4.3
作者：
de Hoon, Michiel J L;Makita, Yuko;Nakai, Kenta;Miyano, Satoru
通讯作者：
Miyano, Satoru