Expression of Mipu1 in response to myocardial infarction in rats.
Expression of Mipu1 in response to myocardial infarction in rats.
复制标题
Mipu1在大鼠心肌梗死中的表达
DOI：
10.3390/ijms10020492
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发表时间：
2009-02
影响因子：
5.6
通讯作者：
Xiao X
中科院分区：
文献类型：
--
作者：
Wang G;Zuo X;Liu J;Jiang L;Liu Y;Zheng Y;Zhang B;Xiao X
本实验室克隆了心肌缺血预适应上调蛋白1（Mipu 1）。雄性Wistar大鼠结扎左冠状动脉前支，假手术后1 h、3 h、6 h、12 h、24 h、3 d、5 d处死。采用北方印迹、实时荧光定量RT-PCR、原位杂交和Western印迹检测Mipu 1 mRNA和蛋白的表达。Mipu 1的表达在3 h上调，持续至12 h，6 h达到高峰。Mipu 1 mRNA和蛋白信号在正常和梗死心肌的内皮和心肌中均有表达，主要表达于梗死区。荧光免疫细胞化学显示Mipu 1蛋白定位于H9 c2肌源性细胞的细胞核，并在细胞暴露于H2 O2后上调。这些结果表明，Mipu 1可能在维持血管稳态和保护肌原细胞免受缺血-再灌注或氧化应激损伤中发挥作用。
Myocardial ischemic preconditioning up-regulated protein 1 (Mipu1) was cloned in our laboratory. Male Wistar rats were subjected to left anterior coronary artery ligation and sham-operation and sacrificed at 1 h, 3 h, 6 h, 12 h, 24 h, 3 d or 5 d after ligation. Expression of Mipu1 mRNA and protein were assessed by Northern blotting, real-time quantitative RT-PCR, In Situ hybridization and Western blotting. Expression of Mipu1 was up-regulated at 3 h and lasted to 12 h with a peak at 6 h. Mipu1 mRNA and protein signals express in the endothelium and myocardium in normal and infarcted heart, mainly in infarcted zone. Fluorescent immunocytochemistry showed that Mipu1 protein was localized to the nuclei of H9c2 myogenic cells and was upregulated after the cells being exposed to H2O2. These observations indicates that Mipu1 may play a role in maintaining vascular homeostasis and protecting the myogenic cells from being injured by ischemia-reperfusion or oxidation stress.
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影响因子：
9.5
作者：
Suzuki, K;Sawa, Y;Matsuda, H
通讯作者：
Matsuda, H
影响因子：
56.9
作者：
YANAGISAWAMIWA, A;UCHIDA, Y;ITO, H
通讯作者：
ITO, H
DOI：
10.1152/ajpheart.1996.270.5.h1803
发表时间：
1996-05-01
影响因子：
4.8
作者：
Li, J;Brown, LF;Simons, M
通讯作者：
Simons, M
影响因子：
5
作者：
Plumier, JCL;Robertson, HA;Currie, RW
通讯作者：
Currie, RW
新型 KRAB/C2H2 锌指蛋白 Mipu1 的功能分析。
DOI：
10.1016/j.bbrc.2007.02.138
发表时间：
2007-05-18
影响因子：
3.1
作者：
Jiang, Lei;Tang, Daolin;Xiao, Xianzhong
通讯作者：
Xiao, Xianzhong