Functional dissection of the ash2 and ash1 transcriptomes provides insights into the transcriptional basis of wing phenotypes and reveals conserved protein interactions.
Functional dissection of the ash2 and ash1 transcriptomes provides insights into the transcriptional basis of wing phenotypes and reveals conserved protein interactions.
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ASH2和ASH1转录组的功能解剖提供了对机翼表型的转录基础的见解，并揭示了保守的蛋白质相互作用。
DOI：
10.1186/gb-2007-8-4-r67
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发表时间：
2007
期刊：
影响因子：
12.3
通讯作者：
Corominas M
中科院分区：
文献类型：
--
作者：
Beltran S;Angulo M;Pignatelli M;Serras F;Corominas M
对来自ash 2和ash 1突变体的翅成虫盘的基因表达谱的分析表明，它们是高度相似的，支持它们一起作用以维持转录的稳定状态的模型。三胸组（trxG）基因缺失，小或同源异形盘1（ash 1）和2（ash 2）中分离的突变体异常的成虫盘的筛选。任何一个基因的突变都会引起同源异型转化，但Hox基因并不是它们唯一的靶基因。尽管对双突变体的分析显示，ash2和ash1突变增强了彼此的表型，表明它们在功能上是相关的，但这些蛋白质是不同复合物的亚基。对ash 2和ash 1突变体的翅成虫盘转录组的分析表明，它们高度相似。使用基因本体论的调节基因的功能注释允许鉴定严重受影响的基因组，其可以与观察到的翅表型相关。与其他全基因组分析的差异表达基因的比较揭示了ASH2和Sin3A之间的相似性，表明了推定的功能关系。多线染色体上的免疫共沉淀研究和免疫定位表明，ASH2和Sin3A与HCF（宿主细胞因子）相互作用。核小体蛋白质印迹和克隆分析结果表明，ASH2是组蛋白3（H3K4）上赖氨酸4三甲基化所必需的。ash 2和ash 1突变体的转录组之间的相似性支持了两个基因共同作用以维持转录稳定状态的模型。与人类一样，ASH2和Sin3A都与HCF结合。最后，在ash2突变体中H3K4三甲基化的减少是果蝇中关于该trxG基因的分子功能的第一个证据。
Analysis of the gene expression profiles of wing imaginal discs from ash2 and ash1 mutants shows that they are highly similar, supporting a model in which they act together to maintain stable states of transcription. The trithorax group (trxG) genes absent, small or homeotic discs 1 (ash1) and 2 (ash2) were isolated in a screen for mutants with abnormal imaginal discs. Mutations in either gene cause homeotic transformations but Hox genes are not their only targets. Although analysis of double mutants revealed that ash2 and ash1 mutations enhance each other's phenotypes, suggesting they are functionally related, it was shown that these proteins are subunits of distinct complexes. The analysis of wing imaginal disc transcriptomes from ash2 and ash1 mutants showed that they are highly similar. Functional annotation of regulated genes using Gene Ontology allowed identification of severely affected groups of genes that could be correlated to the wing phenotypes observed. Comparison of the differentially expressed genes with those from other genome-wide analyses revealed similarities between ASH2 and Sin3A, suggesting a putative functional relationship. Coimmunoprecipitation studies and immunolocalization on polytene chromosomes demonstrated that ASH2 and Sin3A interact with HCF (host-cell factor). The results of nucleosome western blots and clonal analysis indicated that ASH2 is necessary for trimethylation of the Lys4 on histone 3 (H3K4). The similarity between the transcriptomes of ash2 and ash1 mutants supports a model in which the two genes act together to maintain stable states of transcription. Like in humans, both ASH2 and Sin3A bind HCF. Finally, the reduction of H3K4 trimethylation in ash2 mutants is the first evidence in Drosophila regarding the molecular function of this trxG gene.
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影响因子：
56.9
作者：
Furlong, EEM;Andersen, EC;Scott, MP
通讯作者：
Scott, MP
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
16
作者：
Hughes, CM;Rozenblatt-Rosen, O;Meyerson, M
通讯作者：
Meyerson, M
DOI：
10.1073/pnas.0538075100
发表时间：
2003-03-18
影响因子：
11.1
作者：
Beltran, S;Blanco, E;Corominas, M
通讯作者：
Corominas, M
影响因子：
10.5
作者：
Goodliffe, JM;Wieschaus, E;Cole, MD
通讯作者：
Cole, MD