Molecular signatures of tissue-specific microvascular endothelial cell heterogeneity in organ maintenance and regeneration.
Molecular signatures of tissue-specific microvascular endothelial cell heterogeneity in organ maintenance and regeneration.
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DOI：
10.1016/j.devcel.2013.06.017
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发表时间：
2013-07-29
影响因子：
11.8
通讯作者：
Rafii, Shahin
中科院分区：
文献类型：
--
作者：
Nolan, Daniel J.;Ginsberg, Michael;Israely, Edo;Palikuqi, Brisa;Poulos, Michael G.;James, Daylon;Ding, Bi-Sen;Schachterle, William;Liu, Ying;Rosenwaks, Zev;Butler, Jason M.;Xiang, Jenny;Rafii, Arash;Shido, Koji;Rabbany, Sina Y.;Elemento, Olivier;Rafii, Shahin
不同组织中的微血管内皮细胞（ECs）具有不同但尚未被认识的结构、表型和功能属性。我们设计了内皮细胞纯化、培养、分析和移植模型，建立了缺乏淋巴内皮细胞或实质细胞的内皮细胞的组织特异性分子文库。这些库识别赋予ec有机型特征的属性。我们发现转录因子簇、血管分泌生长因子、粘附分子和趋化因子在每个器官的内皮细胞中以独特的组合表达。此外，内皮细胞在组织再生模型、肝切除术和骨髓切除术中有明显的反应。为了测试数据集，我们开发了一个移植模型，使用从胚胎干细胞分化的通用ECs。移植的异型内皮细胞移植到再生组织中，获得器官型内皮细胞的特征。总的来说，我们展示了EC信息数据库在揭示定义EC异质性的血管外和内在信号方面的实用性。这些因素可用于治疗性地设计组织特异性内皮细胞进行再生。
Microvascular endothelial cells (ECs) within different tissues are endowed with distinct but as yet unrecognized structural, phenotypic, and functional attributes. We devised EC purification, cultivation, profiling, and transplantation models that establish tissue-specific molecular libraries of ECs devoid of lymphatic ECs or parenchymal cells. These libraries identify attributes that confer ECs with their organotypic features. We show that clusters of transcription factors, angiocrine growth factors, adhesion molecules, and chemokines are expressed in unique combinations by ECs of each organ. Furthermore, ECs respond distinctly in tissue regeneration models, hepatectomy, and myeloablation. To test the data set, we developed a transplantation model that employs generic ECs differentiated from embryonic stem cells. Transplanted generic ECs engraft into regenerating tissues and acquire features of organotypic ECs. Collectively, we demonstrate the utility of informational databases of ECs toward uncovering the extravascular and intrinsic signals that define EC heterogeneity. These factors could be exploited therapeutically to engineer tissue-specific ECs for regeneration.
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影响因子：
82.9
作者：
通讯作者：
--
影响因子：
64.5
作者：
Ginsberg M;James D;Ding BS;Nolan D;Geng F;Butler JM;Schachterle W;Pulijaal VR;Mathew S;Chasen ST;Xiang J;Rosenwaks Z;Shido K;Elemento O;Rabbany SY;Rafii S
通讯作者：
Rafii S
影响因子：
2.5
作者：
Alva, JA;Zovein, AC;Iruela-Arispe, ML
通讯作者：
Iruela-Arispe, ML
DOI：
10.1083/jcb.200901129
发表时间：
2009-05-18
期刊：
The Journal of cell biology
影响因子：
--
作者：
Goldoni S;Humphries A;Nyström A;Sattar S;Owens RT;McQuillan DJ;Ireton K;Iozzo RV
通讯作者：
Iozzo RV
影响因子：
2.6
作者：
Brauer, PR;Cai, DH
通讯作者：
Cai, DH