Long-term imaging of mouse embryos using adaptive harmonic generation microscopy.

Long-term imaging of mouse embryos using adaptive harmonic generation microscopy.
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DOI:
10.1117/1.3569614
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发表时间:
2011-04
影响因子:
3.5
通讯作者:
Booth M
Booth M
中科院分区:
医学3区
文献类型:
--
作者:
Thayil A;Watanabe T;Jesacher A;Wilson T;Srinivas S;Booth M

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我们提出了一种自适应谐波产生(HG)显微镜和培养技术,允许长期的,小鼠胚胎的三维成像的详细描述。植入前和植入后(0.5-5.5日龄)小鼠胚胎的HG信号被完全表征。第二张HG图像显示细胞分裂过程中的中央纺锤体,而第三张HG图像显示一些特征,如脂滴、核仁和质膜。在为期一天的不连续HG成像中,发现胚胎发育正常,允许观察几个动态事件,如桑葚胚压实和囊胚形成。
We present a detailed description of an adaptive harmonic generation (HG) microscope and culture techniques that permit long-term, three-dimensional imaging of mouse embryos. HG signal from both pre- and postimplantation stage (0.5–5.5 day-old) mouse embryos are fully characterized. The second HG images reveal central spindles during cytokinesis whereas third HG images show several features, such as lipid droplets, nucleoli, and plasma membranes. The embryos are found to develop normally during one-day-long discontinuous HG imaging, permitting the observation of several dynamic events, such as morula compaction and blastocyst formation.
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