Mutations that alter a repeated ACCA element located at the 5' end of the Potato virus X genome affect RNA accumulation.
Mutations that alter a repeated ACCA element located at the 5' end of the Potato virus X genome affect RNA accumulation.
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改变位于马铃薯病毒 X 基因组 5 端的重复 ACCA 元件的突变会影响 RNA 积累。
DOI：
10.1016/j.virol.2008.05.004
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发表时间：
2008
期刊：
影响因子：
3.7
通讯作者：
Kim,Kook-Hyung
中科院分区：
文献类型：
--
作者：
Park,Mi-Ri;Kwon,Sun-Jung;Choi,Hong-Soo;Hemenway,CynthiaL;Kim,Kook-Hyung
马铃薯X病毒（Potato virus X，PVX）RNA的5′非翻译区（NTR）中的ACCA或富含AC的序列和结构（SL 1）元件通过控制翻译、RNA复制、移动和组装在PVX的生命周期中起着重要作用。已经表明，重复的ACCA或富含AC的序列影响gRNA和sgRNA积累，而不影响负链RNA积累，并且也是宿主蛋白结合所需的。通过分析本氏烟草（Nicotiana benthamiana）和NT 1原生质体中PVX基因缺失和定点突变对PVX复制的影响，研究了5′ NTR区ACCA重复序列元件的功能意义。在PVX RNA的5′ NTR的第一个元件（nt 10-13）中引入的取代（ACCA变为AAAA或UUUU）突变显著影响病毒复制，而在第二个元件（nt 17-20）和第三个元件（nt 20-23）中引入的突变则不影响病毒复制。第四个（nt 29-32）ACCA元件对病毒复制的影响很弱，而第五个（nt 38-41）的突变由于AAAA和/或UUUU取代导致SL 1的结构破坏而显著降低病毒复制。对第一个ACCA元件的进一步表征表明，与野生型相比，在nt 10-13处ACCA的重复（nt 10-17，ACCAACCA）导致严重的症状发展，而单个元件（nt 10-13）的缺失（ΔACCA）或该元件的三倍导致症状发展减少。引入第一个ACCA元件的单核苷酸和双核苷酸取代揭示了位于nt位置11和12的CC的重要性。总之，这些结果表明第一个ACCA元件对于PVX复制是重要的。
The repeated ACCA or AC-rich sequence and structural (SL1) elements in the 5′ non-translated region (NTR) of the Potato virus X (PVX) RNA play vital roles in the PVX life cycle by controlling translation, RNA replication, movement, and assembly. It has already been shown that the repeated ACCA or AC-rich sequence affect both gRNA and sgRNA accumulation, while not affecting minus-strand RNA accumulation, and are also required for host protein binding. The functional significance of the repeated ACCA sequence elements in the 5′ NTR region was investigated by analyzing the effects of deletion and site-directed mutations on PVX replication in Nicotiana benthamiana plants and NT1 protoplasts. Substitution (ACCA into AAAA or UUUU) mutations introduced in the first (nt 10–13) element in the 5′ NTR of the PVX RNA significantly affected viral replication, while mutations introduced in the second (nt 17–20) and third (nt 20–23) elements did not. The fourth (nt 29–32) ACCA element weakly affected virus replication, whereas mutations in the fifth (nt 38–41) significantly reduced virus replication due to the structure disruption of SL1 by AAAA and/or UUUU substitutions. Further characterization of the first ACCA element indicated that duplication of ACCA at nt 10–13 (nt 10–17, ACCAACCA) caused severe symptom development as compared to that of wild type, while deletion of the single element (nt 10–13), ΔACCA) or tripling of this element caused reduced symptom development. Single- and double-nucleotide substitutions introduced into the first ACCA element revealed the importance of CC located at nt positions 11 and 12. Altogether, these results indicate that the first ACCA element is important for PVX replication.
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影响因子：
3.5
作者：
DOLJA, VV;GRAMA, DP;ATABEKOV, JG
通讯作者：
ATABEKOV, JG
马铃薯病毒 X RNA 积累所需的茎环结构的恢复表明对错配和 GNRA 四环的选择。
DOI：
10.1006/viro.1999.9843
发表时间：
1999
期刊：
Virology.
影响因子：
--
作者：
Miller,ED;Kim,KH;Hemenway,C
通讯作者：
Hemenway,C
影响因子：
3.7
作者：
HEMENWAY, C;WEISS, J;TUMER, NE
通讯作者：
TUMER, NE
影响因子：
2.3
作者：
Ko, Sug-Ju;Lee, Yong-Hwan;Kim, Kook-Hyung
通讯作者：
Kim, Kook-Hyung
马铃薯病毒 X 基因组 5 区域的茎环结构是正链 RNA 积累所需的。
DOI：
10.1006/jmbi.1998.2174
发表时间：
1998
期刊：
Journal of molecular biology.
影响因子：
--
作者：
Miller,ED;Plante,CA;Kim,KH;Brown,JW;Hemenway,C
通讯作者：
Hemenway,C