Programmed cell death (apoptosis) in lymphoid and myeloid cell lines during zinc deficiency
Programmed cell death (apoptosis) in lymphoid and myeloid cell lines during zinc deficiency
复制标题
缺锌期间淋巴和骨髓细胞系的程序性细胞死亡（细胞凋亡）
DOI：
--
复制
发表时间：
1991
影响因子：
4.6
通讯作者：
B. M. Hannigan
中科院分区：
文献类型：
--
作者：
S. Martin;G. Mazdai;J. Strain;T. Cotter;B. M. Hannigan
在锌充足或缺锌条件下，体外维持三种淋巴（Molt-3和Raji）或骨髓（HL-60）来源的人细胞系。在这些条件下，评估细胞增殖、活力和死亡模式（凋亡或坏死）。所有这三种细胞类型的增殖能力和活力下降的条件下，缺锌。HL-60和Raji培养物中的细胞死亡主要通过凋亡发生，而缺锌Molt-3培养物中的大多数细胞通过坏死死亡。HL-60和Raji细胞缺锌培养物中的细胞凋亡特征为培养物活力缓慢下降，因为出现了具有浓缩和片段化核DNA的细胞。这些形态学变化伴随着细胞浮力密度的增加，这允许通过梯度密度梯度将存活的凋亡细胞与其非凋亡对应物分离。缺锌Molt-3培养物中的坏死特征在于细胞培养物活力的快速丧失，因为这些细胞经历直接裂解。完整的坏死细胞很容易通过其染色质的絮凝状态以及其细胞质的嗜碱性粒细胞减少来识别。对凋亡HL-60和Raji细胞的DNA分析显示，发生了核小体间DNA降解，表明内源性核酸内切酶活化，而坏死Molt-3细胞的核DNA保持相对完整。诱发的细胞死亡的不同模式可能反映了这些谱系的细胞对体内锌水平的相对敏感性。
Three human cell lines of lymphoid (Molt‐3 and Raji) or myeloid (HL‐60) origin were maintained in vitro under zinc‐sufficient or zinc‐deficient conditions. Under these conditions, cell proliferation, viability and mode of death (apoptotic or necrotic) were assessed. All three cell types decreased their proliferative capacity and viability under conditions of zinc deficiency. Cell death in the HL‐60 and Raji cultures occurred primarily via apoptosis, while most cells in zinc‐deficient Molt‐3 cultures died via necrosis. Apoptosis in zinc‐deficient cultures of HL‐60 and Raji cells was characterized by a slow decline in culture viability as cells with condensed and fragmented nuclear DNA appeared. These morphological changes were accompanied by an increase in cell buoyant density, which allowed separation of viable apoptotic cells from their non‐apoptotic counterparts by means of percoll step‐density gradients. Necrosis in zinc‐deficient Molt‐3 cultures was characterized by rapid loss of cell culture viability as these cells underwent direct lysis. Intact necrotic cells were easily identified by the flocculated state of their chromatin as well as the decreased basophilia of their cytoplasm. Analysis of DNA from apoptotic HL‐60 and Raji cells revealed that internucleosomal DNA degradation, indicative of endogenous endonuclease activation, had occurred, whereas the nuclear DNA of necrotic Molt‐3 cells remained relatively unfragmented. The different modes of cell death evoked may reflect the relative sensitivities of cells of these lineages to zinc levels in vivo.
登录
查看更多内容
DOI：
10.1073/pnas.80.20.6361
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DUKE, RC;CHERVENAK, R;COHEN, JJ
通讯作者：
COHEN, JJ
伽马射线照射的基因诱导导致淋巴细胞中的 DNA 断裂。
DOI：
--
发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Sellins,KS;Cohen,JJ
通讯作者：
Cohen,JJ
在胶原蛋白上生长的兔子宫内膜细胞的原代培养物中调节细胞死亡和细胞增殖的可溶性因子的证据。
DOI：
10.1073/pnas.83.13.4784
发表时间：
1986
影响因子：
11.1
作者：
Lynch,MP;Nawaz,S;Gerschenson,LE
通讯作者：
Gerschenson,LE
CTL 靶标中的 DNA 片段化：免疫系统中程序性细胞死亡的一个例子。
影响因子：
--
作者：
Cohen,JJ;Duke,RC;Chervenak,R;Sellins,KS;Olson,LK
通讯作者：
Olson,LK
T 细胞和 B 细胞有丝分裂发生对体外缺锌的不同敏感性。
影响因子：
4.3
作者：
Zanzonico,P;Fernandes,G;Good,RA
通讯作者：
Good,RA