A novel experimental mouse model of peritoneal dissemination of human gastric cancer cells: analysis of the mechanism of peritoneal dissemination using cDNA macroarrays.
A novel experimental mouse model of peritoneal dissemination of human gastric cancer cells: analysis of the mechanism of peritoneal dissemination using cDNA macroarrays.
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DOI：
10.1111/j.1349-7006.2001.tb01157.x
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发表时间：
2001-07
期刊：
影响因子：
--
通讯作者：
Hirata K
中科院分区：
文献类型：
--
作者：
Nomura H;Nishimori H;Yasoshima T;Hata F;Sogahata K;Tanaka H;Nakajima F;Ikeda S;Kamiguchi K;Isomura H;Sato N;Denno R;Hirata K
我们建立了一种新的细胞系NUGC-3 P4 T，在裸鼠中具有高腹膜转移播散潜力。NUGC-3 P4 T细胞来源于人胃癌细胞系NUGC-3，其腹膜播散能力较低。NUGC-3 P4 T细胞在10/10（100%）小鼠中发生腹膜播散，而亲本NUGC-3细胞在1/5（20.0%）小鼠中发生播散。腹膜中的转移灶显示出与亲代细胞诱导的转移灶基本相同的组织学外观。NUGC-3 P4 T细胞的致瘤性、运动活性和对层粘连蛋白的粘附活性均强于NUGC-3细胞。NUGC-3 P4 T中IL-8的产生显著高于NUGC-3。cDNA宏阵列分析显示，与NUGC-3细胞相比，NUGC-3 P4 T细胞中多种细胞因子、白细胞介素和其他免疫调节剂及其受体在mRNA水平上上调或下调。因此，这种独特的细胞系和体内模型可能是有用的，以研究人类胃癌腹膜播散的生物学。
We established a new cell line, NUGC‐3P4T, with high peritoneal metastatic disseminating potential in nude mice. NUGC‐3P4T cells were derived from the human gastric carcinoma line NUGC‐3, which has low capacity for peritoneal dissemination. NUGC‐3P4T cells developed peritoneal dissemination in 10/10 (100%) mice, whereas the parental NUGC‐3 cells developed dissemination in 1/5 (20.0%) mice. The metastatic foci in the peritoneum showed essentially the same histological appearance as those induced by parental cells. The tumorigenicity, the motile activity and the adhesive activity to the laminin of NUGC‐3P4T cells were stronger than those of NUGC‐3 cells. Production of IL‐8 was significantly higher in NUGC‐3P4T than in NUGC‐3. cDNA macroarrays analysis showed that a variety of cytokines, interleukins, and other immunomodulators and their receptors were up‐ or down‐regulated at the mRNA level in NUGC‐3P4T cells, compared with NUGC‐3 cells. Thus, this unique cell line and in vivo model might be useful to study the biology of peritoneal dissemination of human gastric cancer.
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DOI：
10.1111/j.1349-7006.1999.tb00674.x
发表时间：
1999-01
期刊：
Japanese journal of cancer research : Gann
影响因子：
--
作者：
Igarashi N;Kubota T;Otani Y;Matsuzaki SW;Watanabe M;Teramoto T;Kumai K;Tamaki K;Tanzawa K;Kobayashi T;Kitajima M
通讯作者：
Kitajima M
影响因子：
8.8
作者：
Tsujimoto, H.;Takahashi, T.;Hagiwara, A.;Shimotsuma, M.;Sakakura, C.;Osaki, K.;Sasaki, S.;Shirasu, M.;Sakakibara, T.;Ohyama, T.;Sakuyama, A.;Ohgaki, M.;Imanishi, T.;Yamasaki, J.
通讯作者：
Yamasaki, J.
DOI：
10.1093/jnci/82.24.1890
发表时间：
1990-12-19
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
NAKAJIMA, M;MORIKAWA, K;FIDLER, IJ
通讯作者：
FIDLER, IJ
影响因子：
8.8
作者：
Nishimura, S;Chung, Y S;Yashiro, M;Inoue, T;Sowa, M
通讯作者：
Sowa, M
DOI：
10.1073/pnas.88.20.9345
发表时间：
1991-10-01
影响因子：
11.1
作者：
FU, XY;BESTERMAN, JM;HOFFMAN, RM
通讯作者：
HOFFMAN, RM