Learning-related changes in cellular activity within mouse dentate gyrus during trace eyeblink conditioning.
Learning-related changes in cellular activity within mouse dentate gyrus during trace eyeblink conditioning.
复制标题
DOI：
10.1002/hipo.23468
复制
发表时间：
2022-10
期刊：
影响因子：
3.5
通讯作者：
Disterhoft, John F.
中科院分区：
文献类型：
--
作者：
Miller, Lisa N.;Weiss, Craig;Disterhoft, John F.
由于齿状回是海马三突触回路中信息处理的第一个部位，因此它是形成联想记忆的重要结构。先前在兔子身上的发现记录了齿状回内的细胞群，这些细胞可能在痕迹眨眼条件反射（一种联想学习任务）期间桥接刺激之间的时间间隙以支持记忆形成。然而，这项先前的工作无法确定表现出这种活性的细胞类型。为了进一步探索这些变化，我们结合细胞类型的生理测定进行了体内单神经元记录，以研究颗粒细胞，苔藓细胞和中间神经元在学习过程中在齿状回中的功能作用。在对微量眨眼条件反射（一种海马依赖性时间联想记忆任务）进行训练期间，在年轻成年小鼠中进行四极记录。条件反射小鼠能够成功地学习任务，雄性小鼠的学习速度比雌性小鼠快。在条件组中，颗粒细胞往往表现出增加的放电率在条件刺激呈现，而苔藓细胞表现出减少的放电率在跟踪间隔和非条件刺激。有趣的是，中间神经元群体表现出与学习相关的活动增加和减少，这些活动从条件刺激开始并持续到跟踪间隔。目前的研究还发现，在条件反射动物的刺激呈现过程中，θ功率显着增加，并且这种θ功率的变化随着时间的推移而减少。最终，这些数据表明，独特的参与颗粒细胞，苔藓细胞，和中间神经元在齿状回的痕迹联想记忆的形成。这项工作扩展了我们对齿状回功能的了解，有助于了解衰老和疾病如何破坏这一过程。
Because the dentate gyrus serves as the first site for information processing in the hippocampal trisynaptic circuit, it an important structure for the formation of associative memories. Previous findings in rabbit had recorded populations of cells within dentate gyrus that may bridge the temporal gap between stimuli to support memory formation during trace eyeblink conditioning, an associative learning task. However, this previous work was unable to identify the types of cells demonstrating this type of activity. To explore these changes further, we did in vivo single‐neuron recording in conjunction with physiological determination of cell types to investigate the functional role of granule cells, mossy cells, and interneurons in dentate gyrus during learning. Tetrode recordings were performed in young‐adult mice during training on trace eyeblink conditioning, a hippocampal‐dependent temporal associative memory task. Conditioned mice were able to successfully learn the task, with male mice learning at a faster rate than female mice. In the conditioned group, granule cells tended to show an increase in firing rate during conditioned stimulus presentation while mossy cells showed a decrease in firing rate during the trace interval and the unconditioned stimulus. Interestingly, populations of interneurons demonstrated learning‐related increases and decreases in activity that began at onset of the conditioned stimulus and persisted through the trace interval. The current study also found a significant increase in theta power during stimuli presentation in conditioned animals, and this change in theta decreased over time. Ultimately, these data suggest unique involvement of granule cells, mossy cells, and interneurons in dentate gyrus in the formation of a trace associative memory. This work expands our knowledge of dentate gyrus function, helping to discern how aging and disease might disrupt this process.
登录
查看更多内容
影响因子：
5.3
作者：
Bernier, Brian E.;Lacagnina, Anthony F.;Drew, Michael R.
通讯作者：
Drew, Michael R.
腹侧齿状回区域小白蛋白阳性中间神经元的缺陷与小鼠的抑郁样行为有关
影响因子：
15.1
作者：
Chen, Shijia;Chen, Fengpei;Fang, Marong
通讯作者：
Fang, Marong
影响因子：
5.3
作者：
Botterill, Justin J.;Vinod, K. Yaragudri;Scharfman, Helen E.
通讯作者：
Scharfman, Helen E.
影响因子：
2.5
作者：
Buckmaster, PS;Yamawaki, R;Zhang, GF
通讯作者：
Zhang, GF
影响因子：
4.6
作者：
Jung, Dajung;Kim, Soyoun;Royer, Sebastien
通讯作者：
Royer, Sebastien