Growth performance, serum biochemical profile, jejunal morphology, and the expression of nutrients transporter genes in deoxynivalenol (DON)- challenged growing pigs.
Growth performance, serum biochemical profile, jejunal morphology, and the expression of nutrients transporter genes in deoxynivalenol (DON)- challenged growing pigs.
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受脱氧雪腐镰刀菌烯醇 (DON) 攻击的生长猪的生长性能、血清生化特征、空肠形态和营养转运蛋白基因的表达
DOI：
10.1186/s12917-015-0449-y
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发表时间：
2015-07-03
影响因子：
2.6
通讯作者：
Li T
中科院分区：
文献类型：
--
作者：
Wu L;Liao P;He L;Ren W;Yin J;Duan J;Li T
镰刀菌感染与脱氧雪腐镰刀菌醇（DON）的同时生产引起了世界范围内越来越多的饲料安全问题。本研究旨在研究饲粮中不同水平DON对生长猪生长性能、血清生化特征、空肠形态和营养转运基因差异表达的影响。选取24头60日龄健康生长猪（初始体重= 16.3±1.5 kg SE）进行单独饲养，随机分为4组，分别饲喂含0、3、6或12 mg DON/kg饲料，饲喂21 d。对照组与12 mg/kg DON处理组平均日增重（ADG）差异显著，但3 mg/kg DON处理组平均日采食量（ADFI）略高于对照组（P<0.01）。12 mg/kg DON处理组肝脏相对重量极显著高于对照组（P<0.01），其他脏器差异不显著。血清生化方面，3个处理组血清尿素氮（BUN）、碱性磷酸酶（ALP）、丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）值均高于对照组，血清l -缬氨酸、甘氨酸、l -丝氨酸和l -谷氨酰胺浓度均显著降低，尤其是12 mg/kg DON组（P<0.01）。DON污染饲料后血清总超氧化物歧化酶（T-SOD）、谷胱甘肽过氧化物酶（GSH-Px）显著降低（P<0.01）。3种DON污染饲料的绒毛高度显著降低，淋巴细胞数量显著增加（P<0.01）。对照组兴奋性氨基酸转运体-3 （EAAC-3）、钠-葡萄糖转运体-1 （SGLT-1）、二肽转运体-1 （PepT-1）、阳离子氨基酸转运体-1 （CAT-1）和y+ l型氨基酸转运体-1 (lat1) mRNA表达水平均略或显著高于3个DON处理组。这些结果表明含有DON的饲料以剂量依赖的方式引起广泛的影响。由于EAAC-3、SGLT-1、PepT-1、CAT-1和lat1等营养转运基因的选择性调控，这些影响包括体重减轻、活损伤和氧化应激以及营养吸收不良。
Fusarium infection with concurrent production of deoxynivalenol (DON) causes an increasing safety concern with feed worldwide. This study was conducted to determine the effects of varying levels of DON in diets on growth performance, serum biochemical profile, jejunal morphology, and the differential expression of nutrients transporter genes in growing pigs. A total of twenty-four 60-day-old healthy growing pigs (initial body weight = 16.3 ± 1.5 kg SE) were individually housed and randomly assigned to receive one of four diets containing 0, 3, 6 or 12 mg DON/kg feed for 21 days. Differences were observed between control and the 12 mg/kg DON treatment group with regards to average daily gain (ADG), although the value for average daily feed intake (ADFI) in the 3 mg/kg DON treatment group was slightly higher than that in control (P<0.01). The relative liver weight in the 12 mg/kg DON treatment group was significantly greater than that in the control (P<0.01), but there were no significant differences in other organs. With regard to serum biochemistry, the values of blood urea nitrogen (BUN), alkaline phosphatase (ALP), alanine aminotransferase (ALT) and aspartate amino transferase (AST) in the 3 treatment groups were higher than those in the control, and the serum concentrations of L-valine, glycine, L-serine, and L-glutamine were significantly reduced in the 3 treatment groups, especially in the 12 mg/kg DON group (P<0.01). Serum total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px) were markedly decreased after exposure to DON contaminated feeds (P<0.01). The villi height was markedly decreased and the lymphocyte cell numbers markedly increased in the 3 DON contaminated feeds (P<0.01). The mRNA expression levels of excitatory amino acid transporter-3 (EAAC-3), sodium-glucose transporter-1 (SGLT-1), dipeptide transporter-1 (PepT-1), cationic amino acid transporter-1 (CAT-1) and y+L-type amino acid transporter-1 (LAT-1) in control were slightly or markedly higher than those in the 3 DON treatment groups. These results showed that feeds containing DON cause a wide range of effects in a dose-dependent manner. Such effects includes weight loss, live injury and oxidation stress, and malabsorption of nutrients as a result of selective regulation of nutrient transporter genes such as EAAC-3, SGLT-1, PepT-1, CAT-1 and LAT-1.
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影响因子：
3.8
作者：
Amuzie, Chidozie J.;Pestka, James J.
通讯作者：
Pestka, James J.
DOI：
10.1016/0006-291x(91)92067-t
发表时间：
1991-12-31
影响因子：
3.1
作者：
HWANG, ES;HIRAYAMA, BA;WRIGHT, EM
通讯作者：
WRIGHT, EM
影响因子：
2.2
作者：
BERGSJO, B;LANGSETH, W;LARSEN, HJS
通讯作者：
LARSEN, HJS
影响因子：
2.8
作者：
Dinu, Diana;Bodea, Gabriela Oana;Dinischiotu, Anca
通讯作者：
Dinischiotu, Anca
DOI：
10.1080/0003942031000161045
发表时间：
2004-02-01
期刊：
ARCHIVES OF ANIMAL NUTRITION-ARCHIV FUR TIERERNAHRUNG
影响因子：
--
作者：
Dänicke, S;Valenta, H;Flachowsky, G
通讯作者：
Flachowsky, G