Transcriptome and Metabolite Changes during Hydrogen Cyanamide-Induced Floral Bud Break in Sweet Cherry.
Transcriptome and Metabolite Changes during Hydrogen Cyanamide-Induced Floral Bud Break in Sweet Cherry.
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DOI：
10.3389/fpls.2017.01233
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发表时间：
2017
影响因子：
5.6
通讯作者：
Sánchez-Pérez R
中科院分区：
文献类型：
--
作者：
Ionescu IA;López-Ortega G;Burow M;Bayo-Canha A;Junge A;Gericke O;Møller BL;Sánchez-Pérez R
多年生木本植物的芽休眠释放是一个温度依赖过程，因此这些物种的开花受气候变化的影响很大。温带种植区缺乏寒冷的冬天常常导致开花减少和果实产量低。这可能会在不久的将来减少李子属水果和坚果的供应。为了保持高产，深入了解控制芽休眠释放的分子机制是至关重要的。在这里，我们以甜樱桃（Prunus avium L.）为研究材料研究了这些机制，这种作物通常使用农用化学品氰化氢（HC）来补偿冬季寒冷温度的缺乏并诱导开花。在这项工作中，休眠花蕾喷洒氰化氢，然后进行深度RNA测序，确定了三种主要的表达模式响应HC。这些转录水平的结果通过实时定量聚合酶链反应得到验证，并通过植物激素分析（ABA， SA， IAA， CK，乙烯，JA）得到进一步支持。使用这些方法，我们确定了最上调的途径：细胞分裂素途径，以及茉莉酸和氰化氢途径。我们的研究结果强烈提示了这些代谢产物在芽休眠释放中的诱导作用，并为鉴定芽休眠释放的关键基因提供了敲门砖。
Release of bud dormancy in perennial woody plants is a temperature-dependent process and thus flowering in these species is heavily affected by climate change. The lack of cold winters in temperate growing regions often results in reduced flowering and low fruit yields. This is likely to decrease the availability of fruits and nuts of the Prunus spp. in the near future. In order to maintain high yields, it is crucial to gain detailed knowledge on the molecular mechanisms controlling the release of bud dormancy. Here, we studied these mechanisms using sweet cherry (Prunus avium L.), a crop where the agrochemical hydrogen cyanamide (HC) is routinely used to compensate for the lack of cold winter temperatures and to induce flower opening. In this work, dormant flower buds were sprayed with hydrogen cyanamide followed by deep RNA sequencing, identifying three main expression patterns in response to HC. These transcript level results were validated by quantitative real time polymerase chain reaction and supported further by phytohormone profiling (ABA, SA, IAA, CK, ethylene, JA). Using these approaches, we identified the most up-regulated pathways: the cytokinin pathway, as well as the jasmonate and the hydrogen cyanide pathway. Our results strongly suggest an inductive effect of these metabolites in bud dormancy release and provide a stepping stone for the characterization of key genes in bud dormancy release.
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影响因子：
14.9
作者：
NCBI Resource Coordinators
通讯作者：
NCBI Resource Coordinators
影响因子：
5.1
作者：
Downes, BP;Crowell, DN
通讯作者：
Crowell, DN
影响因子：
7.2
作者：
CHAUDHURY, AM;LETHAM, S;DENNIS, ES
通讯作者：
DENNIS, ES
agriGO：农业界的 GO 分析工具包
影响因子：
14.9
作者：
Du Z;Zhou X;Ling Y;Zhang Z;Su Z
通讯作者：
Su Z
DOI：
10.1080/01140671.2004.9514300
发表时间：
2004-06-01
影响因子：
1.3
作者：
Bound, SA;Jones, KM
通讯作者：
Jones, KM