Genome Comparison of Erythromycin Resistant Campylobacter from Turkeys Identifies Hosts and Pathways for Horizontal Spread of erm(B) Genes.
Genome Comparison of Erythromycin Resistant Campylobacter from Turkeys Identifies Hosts and Pathways for Horizontal Spread of erm(B) Genes.
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DOI：
10.3389/fmicb.2017.02240
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发表时间：
2017
影响因子：
5.2
通讯作者：
Sheppard SK
中科院分区：
文献类型：
--
作者：
Florez-Cuadrado D;Ugarte-Ruiz M;Meric G;Quesada A;Porrero MC;Pascoe B;Sáez-Llorente JL;Orozco GL;Domínguez L;Sheppard SK
弯曲杆菌属的病原体是食源性细菌性胃肠炎的最常见原因。弯曲杆菌病主要由空肠弯曲杆菌和结肠弯曲杆菌引起，通过动物源性食物，特别是家禽传播给人类。与许多病原体一样，弯曲杆菌的抗菌素耐药性正在以惊人的速度增加。红霉素处方是需要抗菌治疗的临床病例的治疗选择，但这受到菌株之间红霉素耐药基因β（B）的移动性的影响。在这里，我们评估了170个弯曲杆菌分离株（133个C。coli和37 C.空肠）。红霉素耐药菌株（n = 85; 81 C. coli和4株C. jejuni）进行了筛选，以确定是否存在先前在火鸡分离物中未鉴定出的p53（B）基因。两个阳性C.结果表明，两株大肠埃希菌对氨基糖苷类和四环素类抗生素的耐药决定簇均为β-内酰胺酶（B）基因，包括aad 9、aadE、aph（2″）-IIIa、aph（3′）-IIIa和泰特（O）基因。比较基因组分析鉴定了来自火鸡的弯曲杆菌、来自猪的猪链球菌和来自人类的屎肠球菌和艰难梭菌之间的相同的B序列。这与定殖火鸡的不同细菌物种之间的多个水平转移事件一致。这个例子强调了跨细菌物种边界传播抗菌素耐药性的可能性，这可能会损害它们在抗菌素治疗中的有效性。
Pathogens in the genus Campylobacter are the most common cause of food-borne bacterial gastro-enteritis. Campylobacteriosis, caused principally by Campylobacter jejuni and Campylobacter coli, is transmitted to humans by food of animal origin, especially poultry. As for many pathogens, antimicrobial resistance in Campylobacter is increasing at an alarming rate. Erythromycin prescription is the treatment of choice for clinical cases requiring antimicrobial therapy but this is compromised by mobility of the erythromycin resistance gene erm(B) between strains. Here, we evaluate resistance to six antimicrobials in 170 Campylobacter isolates (133 C. coli and 37 C. jejuni) from turkeys. Erythromycin resistant isolates (n = 85; 81 C. coli and 4 C. jejuni) were screened for the presence of the erm(B) gene, that has not previously been identified in isolates from turkeys. The genomes of two positive C. coli isolates were sequenced and in both isolates the erm(B) gene clustered with resistance determinants against aminoglycosides plus tetracycline, including aad9, aadE, aph(2″)-IIIa, aph(3′)-IIIa, and tet(O) genes. Comparative genomic analysis identified identical erm(B) sequences among Campylobacter from turkeys, Streptococcus suis from pigs and Enterococcus faecium and Clostridium difficile from humans. This is consistent with multiple horizontal transfer events among different bacterial species colonizing turkeys. This example highlights the potential for dissemination of antimicrobial resistance across bacterial species boundaries which may compromise their effectiveness in antimicrobial therapy.
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影响因子：
3.3
作者：
Méric G;Miragaia M;de Been M;Yahara K;Pascoe B;Mageiros L;Mikhail J;Harris LG;Wilkinson TS;Rolo J;Lamble S;Bray JE;Jolley KA;Hanage WP;Bowden R;Maiden MC;Mack D;de Lencastre H;Feil EJ;Corander J;Sheppard SK
通讯作者：
Sheppard SK
影响因子：
4.9
作者：
ARTHUR, M;ANDREMONT, A;COURVALIN, P
通讯作者：
COURVALIN, P
影响因子：
3.2
作者：
BRISSONNOEL, A;ARTHUR, M;COURVALIN, P
通讯作者：
COURVALIN, P
影响因子：
5.4
作者：
Fyfe, Corey;Grossman, Trudy H.;Sutcliffe, Joyce
通讯作者：
Sutcliffe, Joyce
影响因子：
4.9
作者：
Gibreel, A;Kos, VN;Taylor, DE
通讯作者：
Taylor, DE