Ratiometric fluorescence imaging of cell surface pH by poly(ethylene glycol)-phospholipid conjugated with fluorescein isothiocyanate.
Ratiometric fluorescence imaging of cell surface pH by poly(ethylene glycol)-phospholipid conjugated with fluorescein isothiocyanate.
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DOI：
10.1038/s41598-017-17459-y
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发表时间：
2017-12-13
影响因子：
4.6
通讯作者：
Kanai Y
中科院分区：
文献类型：
--
作者：
Ohgaki R;Teramura Y;Hayashi D;Quan L;Okuda S;Nagamori S;Takai M;Kanai Y
各种生理和病理过程都伴随着细胞外质膜区pH值的变化。因此，在生物科学和医学科学中需要分析细胞表面pH的方法。在这项研究中，我们建立了一种使用聚乙二醇-磷脂（PEG-lipid）作为比率荧光探针核心结构的细胞表面pH成像新方法。PEG-脂质是一种合成的两亲性聚合物，最初开发用于移植治疗中的细胞表面修饰。通过其磷脂部分的疏水烷基链，PEG-脂质在细胞外应用时自发插入质膜并保留在细胞表面。我们已经证明，与异硫氰酸荧光素缀合的PEG-脂质（FITC-PEG-脂质）可以用作在弱碱性和酸性pH之间具有优异的时空分辨率的灵敏的和可逆的细胞表面锚定的pH探针。当考虑其应用于高通量体外测定时，用FITC-PEG-脂质标记细胞表面的非常简单的程序也将是有利的。该研究进一步表明，通过使用PEG-脂质作为生物膜锚定的核心结构，还可以开发用于研究生物膜环境的各种探针。
Various physiological and pathological processes are accompanied with the alteration of pH at extracellular juxtamembrane region. Accordingly, the methods to analyze the cell surface pH have been demanded in biological and medical sciences. In this study, we have established a novel methodology for cell surface pH imaging using poly(ethylene glycol)-phospholipid (PEG-lipid) as a core structure of ratiometric fluorescent probes. PEG-lipid is a synthetic amphiphilic polymer originally developed for the cell surface modification in transplantation therapy. Via its hydrophobic alkyl chains of the phospholipid moiety, PEG-lipid is, when applied extracellularly, spontaneously inserted into the plasma membrane and retained at the surface of the cells. We have demonstrated that the PEG-lipid conjugated with fluorescein isothiocyanate (FITC-PEG-lipid) can be used as a sensitive and reversible cell-surface-anchored pH probe between weakly alkaline and acidic pH with an excellent spatiotemporal resolution. The remarkably simple procedure for cell-surface labeling with FITC-PEG-lipid would also be advantageous when considering its application to high-throughput in vitro assay. This study further indicates that various probes useful for the investigation of juxtamembrane environments could also be developed by using PEG-lipid as the core structure for bio-membrane anchoring.
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影响因子：
2.9
作者：
Hashim, Arig Ibrahim;Zhang, Xiaomeng;Wojtkowiak, Jonathan W.;Martinez, Gary V.;Gillies, Robert J.
通讯作者：
Gillies, Robert J.
DOI：
10.1073/pnas.1608247113
发表时间：
2016-07-19
影响因子：
11.1
作者：
Anderson, Michael;Moshnikova, Anna;Andreev, Oleg A.
通讯作者：
Andreev, Oleg A.
影响因子：
6.1
作者：
DIEHL, H
通讯作者：
DIEHL, H
基于半花青的高分辨率比率近红外荧光探针，用于监测体内 pH 值变化
影响因子：
7.4
作者：
Li, Yinhui;Wang, Yijun;Yang, Ronghua
通讯作者：
Yang, Ronghua
影响因子：
14
作者：
Miura, Suguru;Teramura, Yuji;Iwata, Hiroo
通讯作者：
Iwata, Hiroo