New poly(amino acid methacrylate) brush supports the formation of well-defined lipid membranes.
New poly(amino acid methacrylate) brush supports the formation of well-defined lipid membranes.
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DOI：
10.1021/la504163s
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发表时间：
2015-03-31
期刊：
影响因子：
--
通讯作者：
Evans SD
中科院分区：
文献类型：
--
作者：
Blakeston AC;Alswieleh AM;Heath GR;Roth JS;Bao P;Cheng N;Armes SP;Leggett GJ;Bushby RJ;Evans SD
利用一种新型的含有两性离子半胱氨酸基的聚甲基丙烯酸甲酯(PCysMA)刷子作为脂质双层的载体。聚合物刷子在下面的硬支撑物和水相之间提供了12纳米厚的缓冲。在中性pH条件下，电刷的Zeta电位为∼−10 mV。通过结合表面分析技术，发现含有25%DOTAP的阳离子囊泡形成了均匀的脂双层。光漂白后的荧光恢复法测得的脂类迁移率的扩散系数为∼1.5μm2 S-1，与在玻璃基片上观察到的脂类双层的扩散系数相当。
A novel poly(amino acid methacrylate) brush comprising zwitterionic cysteine groups (PCysMA) was utilized as a support for lipid bilayers. The polymer brush provides a 12-nm-thick cushion between the underlying hard support and the aqueous phase. At neutral pH, the zeta potential of the PCysMA brush was ∼−10 mV. Cationic vesicles containing >25% DOTAP were found to form a homogeneous lipid bilayer, as determined by a combination of surface analytical techniques. The lipid mobility as measured by FRAP (fluorescence recovery after photobleaching) gave diffusion coefficients of ∼1.5 μm2 s–1, which are comparable to those observed for lipid bilayers on glass substrates.
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影响因子：
2.9
作者：
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通讯作者：
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3.9
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影响因子：
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作者：
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10.8
作者：
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通讯作者：
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