Coronatine-Based Gene Expression Changes Impart Partial Resistance to Fall Armyworm (Spodoptera frugiperda) in Seedling Maize.
Coronatine-Based Gene Expression Changes Impart Partial Resistance to Fall Armyworm (Spodoptera frugiperda) in Seedling Maize.
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基于冠状的基因表达改变了幼苗玉米中秋季虫（Spodoptera frugiperda）的部分抵抗。
作者：
草地贪夜蛾Spodoptera frugiperda（S. frugiperda，Smith）入侵中国，严重威胁玉米生产。探索有效的方法遏制黑恶势力危害的进一步扩大。以郑单958三叶期（V3）玉米幼苗为材料，研究了冠菌素（COR）对玉米抗虫性的影响。frugiperda）在幼苗期。结果表明，用0.05 μM的COR处理玉米，对玉米新孵幼虫有明显的毒杀作用;草地贪夜蛾取食叶最少。通过对激素含量的测定发现，0.05 μM的COR显著提高了玉米叶片脱落酸（阿坝）和茉莉酸（JA）的含量。此外，转录组测序显示，与苯并恶嗪类化合物合成相关的6个基因（ZmBX 1，ZmBX 2，ZmBX 3，ZmBX 4，ZmBX 5和ZmBX 6）的表达上调。九个基因JA信号通路中JA-Zim结构域（JAZ）蛋白的7个基因（ZmZIM 3、ZmZIM 4、ZmZIM 10、ZmZIM 13、ZmZIM 18、ZmZIM 23、ZmZIM 27、ZmZIM 28和ZmZIM 38），阿坝下游反应蛋白A组2C型蛋白磷酸酶（PP 2C）的ZmPRH 19、ZmPRH 18、Zm 00001 d 024732、Zm 00001 d 034109、Zm 00001 d 026269、Zm 00001 d 028574和Zm 00001 d 013220表达下调。研究结果表明，COR能够诱导玉米苗期抗虫因子，显著提高玉米苗期抗虫性，为进一步研究COR提高玉米苗期抗虫性的机理奠定了理论基础，并为COR作为一种环境友好型害虫防治方法的应用提供了数据参考。
In recent years, Spodoptera frugiperda (S. frugiperda, Smith) has invaded China, seriously threatening maize production. To explore an effective method to curb the further expansion of the harm of the S. frugiperda, this experiment used maize seedlings of the Zhengdan 958 three-leaf stage (V3) of maize as the material to study the effect of coronatine (COR) on the ability of maize to resist insects (S. frugiperda) at the seedling stage. The results showed that when maize was sprayed with 0.05 μM COR, the newly incubated larvae of S. frugiperda had the least leaf feeding. It was found that 0.05 μM COR significantly increased the contents of abscisic acid (ABA) and jasmonate (JA) in maize leaves through the determination of hormone content. Moreover, transcriptome sequencing revealed that the expression of six genes (ZmBX1, ZmBX2, ZmBX3, ZmBX4, ZmBX5 and ZmBX6), which are associated with the synthesis of benzoxazinoid, were upregulated. Nine genes (ZmZIM3, ZmZIM4, ZmZIM10, ZmZIM13, ZmZIM18, ZmZIM23, ZmZIM27, ZmZIM28 and ZmZIM38) of JA-Zim Domain (JAZ) protein in the JA signal pathway, and seven genes (ZmPRH19, ZmPRH18, Zm00001d024732, Zm00001d034109, Zm00001d026269, Zm00001d028574 and Zm00001d013220) of ABA downstream response protein Group A Type 2C Protein Phosphatase (PP2C) were downregulated. These results demonstrated that COR could induce anti-insect factors and significantly improve insect resistance in seedling maize, which laid a theoretical foundation for further study of the mechanism of COR improving insect resistance in seedling maize, and provided data references for the use of COR as an environmentally friendly pest control method.
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