Transcriptome dynamic landscape underlying the improvement of maize lodging resistance under coronatine treatment.
Transcriptome dynamic landscape underlying the improvement of maize lodging resistance under coronatine treatment.
复制标题
冠菌素处理提高玉米抗倒伏性的转录组动态图谱。
DOI：
10.1186/s12870-021-02962-2
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发表时间：
2021-04-27
影响因子：
5.3
通讯作者：
Duan L
中科院分区：
文献类型：
--
作者：
Ren Z;Wang X;Tao Q;Guo Q;Zhou Y;Yi F;Huang G;Li Y;Zhang M;Li Z;Duan L
倒伏是影响玉米产量的重要因素之一。株高是决定玉米（Zea mays L.）植株构型的重要因素，在高密度条件下与抗倒性密切相关。冠菌素（Coronatine，COR）是茉莉酸（jasmonic acid，JA）的一种结构类似物，是一种植物毒素。本研究发现，COR作为一种新型植物生长调节剂，能有效抑制玉米杂交种ZD 958和XY 335以及自交系B73伸长过程中的节间生长，降低玉米株高和穗位高度，从而提高玉米抗倒伏能力。为了研究COR处理后节间基因表达的变化，收集了自交系B73在正常条件和COR处理下节间的时空转录组，包括3个不同发育时期节间的3个不同区域（固定区、分生区和伸长区）。描述正常条件下三个区域的基因表达水平，然后与COR处理后的基因表达水平进行比较。共检测到8605个COR-RGs，其中802个基因在节间特异表达。在这些COR-RGs中，分别有614个、870个和2123个仅在固定区、分生区和伸长区表达变化。在不同区域发现的COR-RGs数量和功能均发生了显著变化，表明在这三个区域调控着不同功能的基因。此外，我们还发现80%以上的赤霉素和茉莉酸基因在COR处理下发生了变化。这些数据提供了一个基因表达谱在不同区域的节间发育和COR影响节间伸长的分子机制。提出了一个假定的示意图的节间反应COR处理，它显示了COR影响节间伸长的基本过程。本研究为玉米节间发育的研究提供了有用的资源，并加深了我们对基于株高的COR调控机制的理解。在线版本包含补充材料，可通过10.1186/s12870-021-02962-2获得。
Lodging is one of the important factors causing maize yield. Plant height is an important factor in determining plant architecture in maize (Zea mays L.), which is closely related to lodging resistance under high planting density. Coronatine (COR), which is a phytotoxin and produced by the pathogen Pseudomonas syringae, is a functional and structural analogue of jasmonic acid (JA). In this study, we found COR, as a new plant growth regulator, could effectively reduce plant height and ear height of both hybrids (ZD958 and XY335) and inbred (B73) maize by inhibiting internode growth during elongation, thus improve maize lodging resistance. To study gene expression changes in internode after COR treatment, we collected spatio-temporal transcriptome of inbred B73 internode under normal condition and COR treatment, including the three different regions of internode (fixed, meristem and elongation regions) at three different developmental stages. The gene expression levels of the three regions at normal condition were described and then compared with that upon COR treatment. In total, 8605 COR-responsive genes (COR-RGs) were found, consist of 802 genes specifically expressed in internode. For these COR-RGs, 614, 870, 2123 of which showed expression changes in only fixed, meristem and elongation region, respectively. Both the number and function were significantly changed for COR-RGs identified in different regions, indicating genes with different functions were regulated at the three regions. Besides, we found more than 80% genes of gibberellin and jasmonic acid were changed under COR treatment. These data provide a gene expression profiling in different regions of internode development and molecular mechanism of COR affecting internode elongation. A putative schematic of the internode response to COR treatment is proposed which shows the basic process of COR affecting internode elongation. This research provides a useful resource for studying maize internode development and improves our understanding of the COR regulation mechanism based on plant height. The online version contains supplementary material available at 10.1186/s12870-021-02962-2.
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影响因子：
12.3
作者：
Dixon DP;Lapthorn A;Edwards R
通讯作者：
Edwards R
影响因子：
11.6
作者：
BENSEN, RJ;JOHAL, GS;BRIGGS, SP
通讯作者：
BRIGGS, SP
DOI：
10.1111/pce.12377
发表时间：
2014-10
期刊：
Plant, cell & environment
影响因子：
--
作者：
Ayano M;Kani T;Kojima M;Sakakibara H;Kitaoka T;Kuroha T;Angeles-Shim RB;Kitano H;Nagai K;Ashikari M
通讯作者：
Ashikari M
影响因子：
3.5
作者：
Gao, Xiquan;Starr, James;Kolomiets, Michael
通讯作者：
Kolomiets, Michael
影响因子：
6.2
作者：
Datta, R;Chourey, PS
通讯作者：
Chourey, PS