Fermentative production of enantiomerically pure S-1,2-propanediol from glucose by engineered E. coli strain
Fermentative production of enantiomerically pure S-1,2-propanediol from glucose by engineered E. coli strain
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通过工程大肠杆菌发酵生产对映体纯的 S-1,2-丙二醇
DOI：
10.1007/s00253-015-7034-y
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发表时间：
2016-02
影响因子：
5
通讯作者：
Yanhe Ma
中科院分区：
文献类型：
--
作者：
Lingfeng Zhu;Xiangchen Guan;Nengzhong Xie;Limin Wang;Bo Yu;Yanhe Ma
1,2-丙二醇纯立体异构体（1,2- pdo）可作为合成高附加值特种化学品和手性药品的起始原料。由于1,2- pdo的立体异构体无法通过传统的化学合成方法得到，生物技术方法越来越受到人们的关注。然而，据我们所知，从葡萄糖中直接产生s -1,2- pdo之前还没有报道。在这项研究中，我们展示了一种新的人工途径，将l -乳酸转化为s -1,2- pdo，并将其整合到大肠杆菌菌株BW25113∆poxB的基因组中，同时缺失了葡萄糖分支代谢途径的基因。用凝固芽孢杆菌的l -乳酸脱氢酶代替天然的d -乳酸脱氢酶可提高l -乳酸的产量。甲基乙二醛旁路通路被阻断，以避免合成D-和l -乳酸的外消旋混合物，并防止甲基乙二醛（一种有毒中间体）的积累。为了进一步提高s -1,2- pdo的产率，我们引入了一种新的辅助因子再生体系，将丙酮酸脱羧酶和乙醛辅酶a脱氢酶II结合在一起，同时再生NADH和乙酰辅酶a的辅酶a供体，用于乳酸转化途径。最后，通过破坏主要的碳竞争途径和加强乳酸转化途径，直接从葡萄糖中产生13.7 mM s -1,2- pdo，其对映体纯度为bbb99 %。本研究首次尝试通过葡萄糖直接发酵合成s -1,2- pdo。
The pure stereoisomers of 1,2-propanediol (1,2-PDO) could be used as starting materials to synthesize high value-added specialty chemicals and chiral pharmaceutical products. As the stereoisomers of 1,2-PDO cannot be obtained by traditional chemical synthesis processes, biotechnological processes have gained increasing attention. However, to our knowledge, the production of S-1,2-PDO directly from glucose has not been previously reported. In this study, we demonstrate a novel artificial pathway to convert L-lactic acid to S-1,2-PDO and its integration into the genome of Escherichia coli strain BW25113∆poxB with synchronous deletion of genes responsible for branch metabolic pathways from glucose. L-lactate production was increased by replacing the native D-lactate dehydrogenase with the L-lactate dehydrogenase from Bacillus coagulans. The methylglyoxal bypass pathway was blocked to avoid synthesis of a racemic mixture of D- and L-lactate and prevent the accumulation of methylglyoxal, a toxic intermediate. To further improve the yield of S-1,2-PDO, a novel cofactor regeneration system was introduced by combining pyruvate decarboxylase and acetaldehyde-CoA dehydrogenase II to simultaneously regenerate NADH and the CoA donor of acetyl-CoA for the lactate conversion pathway. Finally, 13.7 mM S-1,2-PDO with >99 % enantiomeric purity was directly produced from glucose by disrupting the major carbon-competing pathways and strengthening the lactate transformation pathway. This study demonstrates the first attempt to synthesize S-1,2-PDO by direct fermentation of glucose.
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影响因子：
4.4
作者：
Wang, Limin;Cai, Yumeng;Yu, Bo
通讯作者：
Yu, Bo
影响因子：
11.4
作者：
Li, Yuan;Wang, Limin;Ma, Yanhe
通讯作者：
Ma, Yanhe
影响因子：
4.4
作者：
K. C. Raj;Lee A. Talarico;L. Ingram;J. Maupin-Furlow
通讯作者：
K. C. Raj;Lee A. Talarico;L. Ingram;J. Maupin-Furlow
DOI：
10.1016/s0021-9258(18)47893-3
发表时间：
1987-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
L. Baldomá;Juan AguilarS
通讯作者：
L. Baldomá;Juan AguilarS
影响因子：
3.9
作者：
I. R. Booth;G. P. Ferguson;Sarah Christine Miller;Chan Li;Banuri Gunasekera;S. Kinghorn
通讯作者：
I. R. Booth;G. P. Ferguson;Sarah Christine Miller;Chan Li;Banuri Gunasekera;S. Kinghorn