Methanogen activity and microbial diversity in Gulf of Cádiz mud volcano sediments.
Methanogen activity and microbial diversity in Gulf of Cádiz mud volcano sediments.
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DOI：
10.3389/fmicb.2023.1157337
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发表时间：
2023
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
加的斯湾是一个构造活跃的大陆边缘，有超过 60 座泥火山 (MV) 记录在案，其中一些与活跃的甲烷 (CH4) 渗漏有关。然而，原核生物在影响 CH4 释放中的作用很大程度上未知。在两次探险（MSM1-3 和 JC10）中，对加的斯湾的 7 艘 MV（Porto、Bonjardim、Carlos Ribeiro、Captain Arutyunov、Darwin、Meknes 和 Mercator）进行了微生物多样性、地球化学和产甲烷活性分析，加上底物修正浆液还测量了潜在的产甲烷作用和甲烷厌氧氧化 (AOM)。这些 MV 沉积物中的原核生物种群和活动是可变的，反映了它们内部和之间的地球化学异质性。许多MV与其参考站点之间也存在显着差异。 SMTZ (0.2–0.5 mbsf) 以下的总体直接细胞数量远低于一般全球深度分布，相当于 100 mbsf 以下的细胞数量。甲基化合物（尤其是甲胺）的产甲烷作用远高于通常占主导地位的底物 H2/CO2 或乙酸盐。此外，50% 的甲基化底物浆液中均产生 CH4，并且所有七个 MV 位点仅产生甲基营养型 CH4。这些泥浆主要由产甲烷球菌（产生纯培养物）和其他 MV 沉积物中发现的原核生物组成。 AOM 出现在一些泥浆中，特别是来自 Captain Arutyunov、Mercator 和 Carlos Ribeiro MV 的泥浆。 MV位点的古菌多样性显示出产甲烷菌和ANME（Methanosarcinales、Methanococcoides和ANME-1）相关序列的存在，并且细菌多样性高于古菌多样性，主要由Atribacterota、Chloroflexota、Pseudomonadota、Planctomycetota、Bacillota和Ca成员组成。 “阿米尼塞南特斯。”进一步的工作对于确定加的斯湾泥火山对全球甲烷和碳循环的全部贡献至关重要。
The Gulf of Cádiz is a tectonically active continental margin with over sixty mud volcanoes (MV) documented, some associated with active methane (CH4) seepage. However, the role of prokaryotes in influencing this CH4 release is largely unknown. In two expeditions (MSM1-3 and JC10) seven Gulf of Cádiz MVs (Porto, Bonjardim, Carlos Ribeiro, Captain Arutyunov, Darwin, Meknes, and Mercator) were analyzed for microbial diversity, geochemistry, and methanogenic activity, plus substrate amended slurries also measured potential methanogenesis and anaerobic oxidation of methane (AOM). Prokaryotic populations and activities were variable in these MV sediments reflecting the geochemical heterogeneity within and between them. There were also marked differences between many MV and their reference sites. Overall direct cell numbers below the SMTZ (0.2–0.5 mbsf) were much lower than the general global depth distribution and equivalent to cell numbers from below 100 mbsf. Methanogenesis from methyl compounds, especially methylamine, were much higher than the usually dominant substrates H2/CO2 or acetate. Also, CH4 production occurred in 50% of methylated substrate slurries and only methylotrophic CH4 production occurred at all seven MV sites. These slurries were dominated by Methanococcoides methanogens (resulting in pure cultures), and prokaryotes found in other MV sediments. AOM occurred in some slurries, particularly, those from Captain Arutyunov, Mercator and Carlos Ribeiro MVs. Archaeal diversity at MV sites showed the presence of both methanogens and ANME (Methanosarcinales, Methanococcoides, and ANME-1) related sequences, and bacterial diversity was higher than archaeal diversity, dominated by members of the Atribacterota, Chloroflexota, Pseudomonadota, Planctomycetota, Bacillota, and Ca. “Aminicenantes.” Further work is essential to determine the full contribution of Gulf of Cádiz mud volcanoes to the global methane and carbon cycles.
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影响因子：
56.9
作者：
Beal, Emily J.;House, Christopher H.;Orphan, Victoria J.
通讯作者：
Orphan, Victoria J.
影响因子：
5
作者：
Etiope, Giuseppe
通讯作者：
Etiope, Giuseppe
影响因子：
5.1
作者：
Webster G;Blazejak A;Cragg BA;Schippers A;Sass H;Rinna J;Tang X;Mathes F;Ferdelman TG;Fry JC;Weightman AJ;Parkes RJ
通讯作者：
Parkes RJ
影响因子：
4.4
作者：
Borrel G;Parisot N;Harris HM;Peyretaillade E;Gaci N;Tottey W;Bardot O;Raymann K;Gribaldo S;Peyret P;O'Toole PW;Brugère JF
通讯作者：
Brugère JF
DOI：
10.1073/pnas.1000080107
发表时间：
2011-03-15
影响因子：
11.1
作者：
Caporaso, J. Gregory;Lauber, Christian L.;Knight, Rob
通讯作者：
Knight, Rob