Intra-Articular Injection of Alginate-Microencapsulated Adipose Tissue-Derived Mesenchymal Stem Cells for the Treatment of Osteoarthritis in Rabbits.
Intra-Articular Injection of Alginate-Microencapsulated Adipose Tissue-Derived Mesenchymal Stem Cells for the Treatment of Osteoarthritis in Rabbits.
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DOI：
10.1155/2018/2791632
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发表时间：
2018
影响因子：
4.3
通讯作者：
Kang BJ
中科院分区：
文献类型：
--
作者：
Choi S;Kim JH;Ha J;Jeong BI;Jung YC;Lee GS;Woo HM;Kang BJ
我们研究了关节内注射藻酸盐微囊化脂肪组织来源的间充质干细胞（ASC）在兔前交叉韧带横断（ACLT）模型骨关节炎（OA）发展过程中的作用。我们通过双侧ACLT在成熟的新西兰白色兔中诱导OA。根据关节内注射材料的不同，将僵硬关节分为4组。采用振动喷嘴技术制备海藻酸钠微球和微囊化ASC。ACLT后两周，兔子接受连续三周的关节内注射0.9%NaCl、藻酸盐微珠、ASC或微囊化ASC到每个关节中。ACLT后9周，我们对家兔实施安乐死，并收集双侧股骨髁进行肉眼、组织学和免疫组织化学分析。使用改良的OA研究学会国际（OARSI）评分和总软骨损伤评分进行的宏观评价显示，微生物学ASC组股骨髁上的软骨退化相对较低。股骨外侧髁的组织学分析表明，微囊化ASC具有显着的软骨保护作用。免疫组化结果显示，微囊化ASC治疗后膝关节软骨损伤后MMP-13的表达相对较低。在实验性OA的发展过程中，与单独的ASC相比，关节内注射微囊化ASC显著降低了OA的进展和程度。
We investigated the effects of intra-articular injections of alginate-microencapsulated adipose tissue-derived mesenchymal stem cells (ASCs) during osteoarthritis (OA) development in a rabbit model of anterior cruciate ligament transection (ACLT). We induced OA in mature New Zealand white rabbits by bilateral ACLT. Stifle joints were categorised into four groups according to intra-articular injection materials. Alginate microbeads and microencapsulated ASCs were prepared using the vibrational nozzle technology. Two weeks after ACLT, the rabbits received three consecutive weekly intra-articular injections of 0.9% NaCl, alginate microbeads, ASCs, or microencapsulated ASCs, into each joint. Nine weeks after ACLT, we euthanised the rabbits and collected bilateral femoral condyles for macroscopic, histological, and immunohistochemical analyses. Macroscopic evaluation using the modified OA Research Society International (OARSI) score and total cartilage damage score showed that cartilage degradation on the femoral condyle was relatively low in the microencapsulated-ASC group. Histological analysis of the lateral femoral condyles indicated that microencapsulated ASCs had significant chondroprotective effects. Immunohistochemically, the expression of MMP-13 after the articular cartilage damage was relatively low in the microencapsulated-ASC-treated stifle joints. During the development of experimental OA, as compared to ASCs alone, intra-articular injection of microencapsulated ASCs significantly decreased the progression and extent of OA.
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影响因子：
3.7
作者：
He X;Liu Y;Yuan X;Lu L
通讯作者：
Lu L
影响因子：
2.3
作者：
Kuroda K;Kabata T;Hayashi K;Maeda T;Kajino Y;Iwai S;Fujita K;Hasegawa K;Inoue D;Sugimoto N;Tsuchiya H
通讯作者：
Tsuchiya H
影响因子：
4.8
作者：
Filardo, Giuseppe;Di Matteo, Berardo;Kon, Elizaveta
通讯作者：
Kon, Elizaveta
DOI：
10.1292/jvms.16-0456
发表时间：
2017-03-18
期刊：
The Journal of veterinary medical science
影响因子：
--
作者：
Koh E;Jung YC;Woo HM;Kang BJ
通讯作者：
Kang BJ
影响因子：
1.8
作者：
Freire, Mila;Brown, James;Lascelles, B. Duncan X.
通讯作者：
Lascelles, B. Duncan X.