A premature stop codon within the tvb receptor gene results in decreased susceptibility to infection by avian leukosis virus subgroups B, D, and E.
A premature stop codon within the tvb receptor gene results in decreased susceptibility to infection by avian leukosis virus subgroups B, D, and E.
复制标题
tvb 受体基因内的过早终止密码子会导致禽白血病病毒 B、D 和 E 亚型感染的易感性降低
DOI：
10.18632/oncotarget.22512
复制
发表时间：
2017-12-01
期刊：
影响因子：
--
通讯作者：
Xie Q
中科院分区：
文献类型：
--
作者：
Chen W;Liu Y;Li A;Li X;Li H;Dai Z;Yan Y;Zhang X;Shu D;Zhang H;Lin W;Ma J;Xie Q
禽白血病病毒（Avian leukosis virus，ALV）是一种可引起鸡多种肿瘤的致癌病毒。鸡中的禽白血病病毒组包含六个密切相关的亚组，A至E和J。宿主中ALV的流行可能对ALV感染的抗性施加了强大的选择压力。tv B基因编码Tv B受体并决定对亚群B、D和E ALV的易感性或抗性。在这项研究中，我们的特点是一种新的耐药等位基因的tvb受体基因，tvbr 3，它携带一个单核苷酸取代（c.298C>T），构成一个提前终止密码子内的第四外显子，并导致生产的截短TvbR 3受体蛋白。结果，我们观察到体外和体内对ALV-B B、ALV-D和ALV-E感染的易感性降低，并且TvbR 3受体对亚群B、D和E ALV包膜糖蛋白的结合亲和力降低。此外，我们发现tvbr 3等位基因在中国肉鸡品系中普遍存在。本研究表明，tvb受体基因中的提前终止密码子可以赋予宿主对B、D和E亚群ALV的遗传抗性，并提示tvbr 3可能作为ALV-B、ALV-D和ALV-E感染的潜在抗性靶点。
Avian leukosis virus (ALV) is an oncogenic virus causing a variety of neoplasms in chickens. The group of avian leukosis virus in chickens contains six closely related subgroups, A to E and J. The prevalence of ALVs in hosts may have imposed strong selective pressure toward resistance to ALVs infection. The tvb gene encodes Tvb receptor and determines susceptibility or resistance to the subgroups B, D, and E ALV. In this study, we characterized a novel resistant allele of the tvb receptor gene, tvbr3, which carries a single-nucleotide substitution (c.298C>T) that constitutes a premature termination codon within the fourth exon and leads to the production of a truncated TvbR3 receptor protein. As a result, we observed decreased susceptibility to infection by ALV-B, ALV-D and ALV-E both in vitro and in vivo, and decreased the binding affinity of the TvbR3 receptor for the subgroups B, D, and E ALV envelope glycoproteins. Additionally, we found that the tvbr3 allele was prevalent in Chinese broiler lines. This study demonstrated that premature termination codon in the tvb receptor gene can confer genetic resistance to subgroups B, D, and E ALV in the host, and indicates that tvbr3 could potentially serve as a resistant target against ALV-B, ALV-D and ALV-E infection.
登录
查看更多内容
影响因子：
9.4
作者：
Gao, Yulong;Yun, Bingling;Wang, Xiaomei
通讯作者：
Wang, Xiaomei
影响因子：
5.4
作者：
Knauss, DJ;Young, JAT
通讯作者：
Young, JAT
影响因子：
4.8
作者：
Dai M;Feng M;Liu D;Cao W;Liao M
通讯作者：
Liao M
自噬靶向 DNA 疫苗针对禽白血病病毒 J 亚型的功效
影响因子：
5.5
作者：
Dai, Zhenkai;Huang, Jianfei;Xie, Qingmei
通讯作者：
Xie, Qingmei
影响因子：
4.4
作者：
Kreager, KS
通讯作者：
Kreager, KS