The Differences in the Susceptibility Patterns to Triclabendazole Sulfoxide in Field Isolates of Fasciola hepatica Are Associated with Geographic, Seasonal, and Morphometric Variations.
The Differences in the Susceptibility Patterns to Triclabendazole Sulfoxide in Field Isolates of Fasciola hepatica Are Associated with Geographic, Seasonal, and Morphometric Variations.
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在筋膜肝的田间分离株中，易感性模式的敏感性模式与地理，季节性和形态计量学变化有关。
DOI：
10.3390/pathogens11060625
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发表时间：
2022-05-28
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影响因子：
--
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中科院分区：
文献类型：
--
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三氯苯达唑 (TCBZ) 耐药性是片形吸虫病中一个新出现的问题，但目前尚不清楚。包括少量寄生虫的研究未能捕捉到肝片形吸虫群体之间和内部易感性差异的复杂性。作为研究复杂耐药表型-基因型关联的第一步，我们对大量具有不同 TCBZ 敏感性的肝镰刀菌成虫样本进行了表征。我们从秘鲁库斯科和卡哈马卡地区屠宰的自然感染牲畜中收集了寄生虫。这些寄生虫在体外暴露于 TCBZ 亚砜 (TCBZ.SO) 以确定其敏感性。我们使用运动评分来确定寄生虫的生存能力。我们滴定了药物浓度和时间，以检测 20% 无法存活（易感条件）或 80% 无法存活（耐药条件）的寄生虫。我们将 3348 种完全能动的寄生虫暴露于易感（n = 1565）或耐药（n = 1783）条件下。 341 例 (21.8%) 被归类为易感，462 例 (25.9%) 被归类为耐药。库斯科发现的耐药寄生虫比卡哈马卡多（p < 0.001）。抗性寄生虫因屠宰场（p < 0.001）、一年中的月份（p = 0.008）、吸虫长度（p = 0.016）和收集年份（p < 0.001）而异。野生型肝镰刀菌对 TCBZ.SO 的体外敏感性与地理、季节和形态相关。
Triclabendazole (TCBZ) resistance is an emerging problem in fascioliasis that is not well understood. Studies including small numbers of parasites fail to capture the complexity of susceptibility variations between and within Fasciola hepatica populations. As the first step to studying the complex resistant phenotype–genotype associations, we characterized a large sample of adult F. hepatica with diverging TCBZ susceptibility. We collected parasites from naturally infected livestock slaughtered in the Cusco and Cajamarca regions of Peru. These parasites were exposed to TCBZ sulfoxide (TCBZ.SO) in vitro to determine their susceptibility. We used a motility score to determine the parasite’s viability. We titrated drug concentrations and times to detect 20% non-viable (susceptible conditions) or 80% non-viable (resistant conditions) parasites. We exposed 3348 fully motile parasites to susceptible (n = 1565) or resistant (n = 1783) conditions. Three hundred and forty-one (21.8%) were classified as susceptible and 462 (25.9%) were classified as resistant. More resistant parasites were found in Cusco than in Cajamarca (p < 0.001). Resistant parasites varied by slaughterhouse (p < 0.001), month of the year (p = 0.008), fluke length (p = 0.016), and year of collection (p < 0.001). The in vitro susceptibility to TCBZ.SO in wildtype F. hepatica was associated with geography, season, and morphometry.
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影响因子：
4.4
作者：
Fuerst, Thomas;Duthaler, Urs;Keiser, Jennifer
通讯作者：
Keiser, Jennifer
影响因子：
1.7
作者：
Arias-Pacheco, Carmen;Raul Lucas, J.;Lux-Hoppe, Estevam G.
通讯作者：
Lux-Hoppe, Estevam G.
影响因子：
2
作者：
Baska, Piotr;Norbury, Luke James;Januszkiewicz, Kamil
通讯作者：
Januszkiewicz, Kamil
影响因子：
5.6
作者：
Brecht, Karin;Kirchhofer, Carla;Kraehenbuehl, Stephan
通讯作者：
Kraehenbuehl, Stephan
影响因子：
2.2
作者：
Cwiklinski K;O'Neill SM;Donnelly S;Dalton JP
通讯作者：
Dalton JP