Structural studies on archaeal phytanyl-ether lipids isolated from membranes of extreme halophiles by linear ion-trap multiple-stage tandem mass spectrometry with electrospray ionization.
Structural studies on archaeal phytanyl-ether lipids isolated from membranes of extreme halophiles by linear ion-trap multiple-stage tandem mass spectrometry with electrospray ionization.
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DOI：
10.1016/j.aca.2013.02.010
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发表时间：
2013-04-10
影响因子：
6.2
通讯作者：
Corcelli A
中科院分区：
文献类型：
--
作者：
Hsu FF;Lobasso S;Turk J;Corcelli A
古细菌甘油磷脂和糖脂的结构是独特的，因为它们由与甘油主链连接的植烷基取代基醚组成，从而赋予分子稳定性。在这篇文章中，我们描述了多级线性离子阱结合高分辨率质谱法对该脂质家族的结构表征，该脂质家族通过ESI解吸为锂化加合物离子或[M-H]−和[M -2 H]2−离子。MSn对各种形式的锂化加合物离子产生丰富的结构信息的离子，导致完整的结构鉴定这个脂质家族，包括植烷链的甲基分支的位置。相比之下，从[M-H]−和[M -2 H]2−离子上的MSn得到的结构信息并不完整。在离子阱中的裂解途径，包括不寻常的内部损失的甘油部分和内部损失的己糖发现这个脂质家族提出。这种质谱方法提供了一种简单的工具，以促进这种独特的脂质家族的可靠表征。
The structures of archaeal glycerophospholipids and glycolipids are unique in that they consist of phytanyl substituents ether linked to the glycerol backbone, imparting stability to the molecules. In this contribution, we described multiple-stage linear ion-trap combined with high resolution mass spectrometry toward structural characterization of this lipid family desorbed as lithiated adduct ions or as the [M –H]− and [M – 2H]2− ions by ESI. MSn on various forms of the lithiated adduct ions yielded rich structurally informative ions leading to complete structure identification of this lipid family, including the location of the methyl branches of the phytanyl chain. By contrast, structural information deriving from MSn on the [M –H]− and [M – 2H]2− ions is not complete. The fragmentation pathways in an ion-trap, including unusual internal loss of glycerol moiety and internal loss of hexose found for this lipid family were proposed. This mass spectrometric approach provides a simple tool to facilitate confident characterization of this unique lipid family.
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DOI：
10.1016/j.jasms.2010.07.003
发表时间：
2010-11
影响因子：
3.2
作者：
Hsu FF;Turk J
通讯作者：
Turk J
DOI：
10.1016/j.jasms.2003.12.007
发表时间：
2004-04-01
影响因子：
3.2
作者：
Hsu, FF;Turk, J
通讯作者：
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影响因子：
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作者：
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DOI：
10.1016/s1044-0305(98)00036-1
发表时间：
1998-07-01
影响因子：
3.2
作者：
Warrack, BM;Hail, ME;Traldi, P
通讯作者：
Traldi, P
DOI：
10.1016/j.jasms.2005.11.019
发表时间：
2006-03-01
影响因子：
3.2
作者：
Hsu, FF;Turk, J
通讯作者：
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