Design and applicability of DNA arrays and DNA barcodes in biodiversity monitoring.
Design and applicability of DNA arrays and DNA barcodes in biodiversity monitoring.
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DOI：
10.1186/1741-7007-5-24
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发表时间：
2007-06-13
期刊：
影响因子：
5.4
通讯作者：
Hebert PD
中科院分区：
文献类型：
--
作者：
Hajibabaei M;Singer GA;Clare EL;Hebert PD
快速准确地识别物种是大规模生物多样性监测计划的关键组成部分。DNA阵列(微观和宏观)和DNA条形码是最近引起广泛关注的两种分子方法。在这里，我们比较这两个平台，以确定一个重要的群体，哺乳动物。我们基于两个常用的线粒体基因细胞色素c氧化酶I(动物物种的标准DNA条形码)和细胞色素b(常见的物种水平标记)进行的分析表明，阵列和条形码都能够高精度地区分哺乳动物物种。我们使用了三个不同的哺乳动物物种数据集，包括不同的采样策略。对于DNA阵列，我们为每个物种设计了三个探针来解决种内差异。至于DNA条形码，我们的分析表明，细胞色素c氧化酶I和细胞色素b基因，甚至它们的更小片段(微型条形码)都可以成功地在各种各样的标本中区分物种。这项研究表明，DNA阵列和DNA条形码是生物多样性监测的有价值的分子方法。这两种方法都能够在我们的测试组合中区分哺乳动物物种。然而，由于设计DNA阵列需要事先了解目标序列，这种方法在可能遇到未知单倍型的大规模监测程序中的使用可能会受到限制。相比之下，DNA条形码是基于测序的，因此可以在大规模研究中提供更大的灵活性。
The rapid and accurate identification of species is a critical component of large-scale biodiversity monitoring programs. DNA arrays (micro and macro) and DNA barcodes are two molecular approaches that have recently garnered much attention. Here, we compare these two platforms for identification of an important group, the mammals. Our analyses, based on the two commonly used mitochondrial genes cytochrome c oxidase I (the standard DNA barcode for animal species) and cytochrome b (a common species-level marker), suggest that both arrays and barcodes are capable of discriminating mammalian species with high accuracy. We used three different datasets of mammalian species, comprising different sampling strategies. For DNA arrays we designed three probes for each species to address intraspecific variation. As for DNA barcoding, our analyses show that both cytochrome c oxidase I and cytochrome b genes, and even smaller fragments of them (mini-barcodes) can successfully discriminate species in a wide variety of specimens. This study showed that DNA arrays and DNA barcodes are valuable molecular methods for biodiversity monitoring programs. Both approaches were capable of discriminating among mammalian species in our test assemblages. However, because designing DNA arrays require advance knowledge of target sequences, the use of this approach could be limited in large scale monitoring programs where unknown haplotypes might be encountered. DNA barcodes, by contrast, are sequencing-based and therefore could provide more flexibility in large-scale studies.
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影响因子：
9.8
作者：
Hebert PD;Stoeckle MY;Zemlak TS;Francis CM
通讯作者：
Francis CM
DOI：
10.1098/rstb.2005.1716
发表时间：
2005-10-29
影响因子：
6.3
作者：
Ward, RD;Zemlak, TS;Hebert, PDN
通讯作者：
Hebert, PDN
影响因子：
10.7
作者：
SAITOU, N;NEI, M
通讯作者：
NEI, M
影响因子：
4.9
作者：
Pfunder, M;Holzgang, O;Frey, JE
通讯作者：
Frey, JE
影响因子：
4.4
作者：
Peplies, Joerg;Lachmund, Christine;Manz, Werner
通讯作者：
Manz, Werner