Stereological analysis of mammalian skeletal muscle. I. Soleus muscle of the adult guinea pig.
Stereological analysis of mammalian skeletal muscle. I. Soleus muscle of the adult guinea pig.
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DOI：
10.1083/jcb.60.3.732
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发表时间：
1974-03
影响因子：
7.8
通讯作者：
PETER, JB
中科院分区：
文献类型：
--
作者：
EISENBERG, BR;KUDA, AM;PETER, JB
用体视学的点数计算法对豚鼠比目鱼肌肌纤维的超微结构成分的体积、表面积和尺寸进行了定量分析。肌肉纤维具有结构取向(各向异性)，并具有纤维内结构的空间梯度；因此，标准体视学方法在必要时进行了修改。整个分析在两个截面方向上重复，以测试修改和从两个方向获得的相同结果。脂滴体积为0.20±0.06%(平均±标准差，n=5)，核体积为0.86±0.20%。线粒体总体积为纤维体积的4.85±0.66%，约1/3位于肌膜1微米以内的环内。剩余核心区线粒体体积为3.6±0.4%。T系统的体积为0.14±0.01%，比表面积为0.064±0.005微米~2/微米~3。肌膜表面积为0.116±0.013微米~2/微米~3，是T系统表面积的两倍。整个肌浆网体积为3.52±0.33%，表面积为0.97±0.09微米~2/微米~3。肌浆网由终池组成，体积为1.04±0.19%，表面积为0.24±0.05微米~2/微米~3。I带和A带肌浆网小管体积分别为1.97±0.24%和0.51±0.08%，I带和A带肌浆网表面积分别为0.56±0.07微米~2/微米~3和0.16±0.04微米2/微米~3。测量Z线宽度、肌原纤维和肌纤维直径。
A quantitative analysis of the volumes, surface areas, and dimensions of the ultrastructural components in the soleus muscle fibers of the guinea pig was made by using point counting methods of stereology. Muscle fibers have structural orientation (anisotropy) and have spatial gradients of the structures within the fiber; therefore the standard stereological methods were modified where necessary. The entire analysis was repeated at two section orientations to test the modifications and identical results obtained from both. The volume of lipid droplets was 0.20 ± 0.06% (mean ± standard error, n = 5 animals) and the nuclei volume was 0.86 ± 0.20% of the fiber volume. The total mitochondrial volume was 4.85 ± 0.66% of the fiber volume with about one-third being found in an annulus within 1 µm of the sarcolemma. The mitochondrial volume in the remaining core of the fiber was 3.6 ± 0.4%. The T system has a volume of 0.14 ± 0.01% and a surface area of 0.064 ± 0.005 µm2/µm3 of the fiber volume. The surface area of the sarcolemma is 0.116 ± 0.013 µm2/µm3 which is twice the T system surface area. The volume of the entire sarcoplasmic reticulum is 3.52 ± 0.33% and the surface area is 0.97 ± 0.09 µm2/µm3. The sarcoplasmic reticulum is composed of the terminal cisternae whose volume is 1.04 ± 0.19% and surface area is 0.24 ± 0.05 µm2/µm3. The tubules of the sarcoplasmic reticulum in the I band and A band have volumes of 1.97 ± 0.24% and 0.51 ± 0.08%, and the surface areas of the I and A band reticulum are 0.56 ± 0.07 µm2/µm3 and 0.16 ± 0.04 µm2/µm3, respectively. The Z line width, myofibril and fiber diameters were measured.
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影响因子：
7.8
作者：
EISENBERG, B;EISENBERG, RS
通讯作者：
EISENBERG, RS
影响因子：
--
作者：
KELLY, DE;CAHILL, MA
通讯作者：
CAHILL, MA
影响因子：
--
作者：
BARNARD, RJ;EDGERTON, VR;PETER, JB
通讯作者：
PETER, JB
影响因子：
3.2
作者：
EDGERTON, VR;SIMPSON, DR
通讯作者：
SIMPSON, DR
DOI：
10.1007/bf00307369
发表时间：
1973-01-01
期刊：
ZEITSCHRIFT FUR ZELLFORSCHUNG UND MIKROSKOPISCHE ANATOMIE
影响因子：
--
作者：
KILARSKI, W
通讯作者：
KILARSKI, W