Asynchronous appearance of newly synthesized histone H1 subfractions in HeLa chromatin.

Asynchronous appearance of newly synthesized histone H1 subfractions in HeLa chromatin.
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DOI:
10.1083/jcb.90.2.415
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发表时间:
1981-08
期刊:
The Journal of cell biology
影响因子:
--
通讯作者:
Cole RD
Cole RD
中科院分区:
其他
文献类型:
--
作者:
Sizemore SR;Cole RD

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在双胸苷阻断技术同步化的HeLa细胞中,研究了放射性丙氨酸掺入H1组蛋白染色质结合亚组分中的情况。亚组分经Biorex-70色谱仪分离为3个色谱峰。在胸苷阻滞释放后5-7 h期间,峰I和III显示的掺入量是释放后1-3 h期间的两倍,而峰II显示的5-7 h掺入量是1- 3 h的3倍。因此,峰II中的H1-组蛋白亚组分在染色质中出现的时间比峰I和III中的亚组分稍晚于S期。
Incorporation of radioactive alanine into chromatin-bound subfractions of H1 histone was studied in HeLa cells synchronized by the double thymidine block technique. The subfractions were resolved into three chromatographic peaks by Biorex-70. In the period 5-7 h after release from the thymidine block, peaks I and III showed twice as much incorporation as they did in the period 1-3 h after release, whereas peak II showed three times the incorporation at 5-7 h that it did at 1- 3 h. Thus, the H1-histone subfraction in peak II appears in chromatin somewhat later in S phase than do the subfractions in Peaks I and III.
DOI: 10.1101/sqb.1978.042.01.105
发表时间: 1977-01-01
期刊: COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY
影响因子: --
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